B.S., Chemistry; Purdue University, 1975

Ph.D., Biochemistry; University of Wisconsin, Madison, 1981

Mark Sullivan, Ph.D.

Research Associate Professor of Biochemistry & Biophysics in the Center for Oral Biology

Primary Appointment:
Biochemistry & Biophysics

Center Affiliation:
Center for Oral Biology

Research:
Phage antibody technology in the development of novel reagents for characterization of protein expression and structures

See the Sullivan Lab homepage for details.

Contact Information:
E-Mail: Mark_Sullivan@urmc.rochester.edu

University of Rochester
School of Medicine and Dentistry
601 Elmwood Ave
Rochester, New York 14642

KMRB G9627
Phone: (585) 273-1629
Fax: (585) 276-0190

Research Overview

The ability to rapidly isolate human antibodies using phage display has opened up new approaches to understanding protein function. Research in my laboratory is focused on the manipulation of immunoglobulin molecules to develop novel reagents for characterization of protein expression and structure. We are using phage antibody technology in several areas of proteomics research to develop improved reagents for protein characterization as well as novel inhibitors of protein function that will aid in defining the roles of the many new proteins discovered through genomic sequencing. Major efforts are aimed at dissecting the saliva proteome and developing panels of antibodies for incorporation into new diagnostic platforms using protein microarrays. A second initiative is to derive antibodies useful for solving the structure of proteins by X-ray crystallography. Additional studies focus on the therapeutic potential of single-chain antibodies to reduce protein aggregation and misfolding, a process that characterizes a variety of neurodegenerative diseases.

Publications

Bliss JM, Sullivan MA, Malone J, Haidaris CG. Differentiation of Candida albicans and Candida dublinensis using recombinant human antibody single chain variable fragments specific for hyphae. J Clin Microbiol 41:1152-1160, 2003

Haidaris CG, Malone J, Sherril LA, Bliss JM, Gaspari AA, Insel RA, Sullivan MA. Recombinant human antibody single chain variable fragments reactive with Candida albicans surface antigens. J Immunol Methods 257:185-202, 2001

Malone J, Sullivan MA. Analysis of antibody selection by phage display utilizing anti-phenobarbital antibodies.  J Mol Recog 9:738-745, 1996

Murali R, Helmer-Critterich M, Sharkey DJ, Scalice ER, Daiss JL, Sullivan MA, Krishna Murthy HM. Structural studies on an inhibitory antibody against Thermus aquaticus DNA polymerase suggest mode of inhibition. Protein Eng 11:79-86, 1998

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