News and Updates

The Human Immunology Center Core Laboratory announces the availability of 11-color sorting on the BD LSR II flow cytometer located in the HIC Lab, MRB 2-9905. The full upgraded configuration is listed below.

Detector Array (Laser) PMT (Detector) Longpass Dichroic Mirror Bandpass Filter Fluorochrome
Octagon (480nm blue laser) A 735LP 780/60 PE-Cy7
B 685LP 695/40 PerCP-Cy5.5, PE-Cy5.5, PerCP, 7AAD
C 665LP* 670/14 PE-Cy5
D 600LP 610/20 PE-TexasRed
E 550LP 576/26 PE
F* 505LP 530/30 FITC
G* blank 488/10 SSC
Violet Trigon (405nm violet laser) A 505LP 525/50 AlexaFluor430, CFP
B blank 440/40 AlexaFluor405, Pacific Blue, Marina Blue
Red Trigon (633nm red laser) A 735LP 780/60 APC-Cy7
B 685LP* 720/40* APC-Cy5.5
C* blank 660/20 APC
* Need: 2PMTs for 488 octagon, 1PMT for 633 Trigon (BD) 685LP, 655LP, 720/40 (Omega)

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