<Mark Sullivan>

B.S., Chemistry; Purdue University, 1975 Ph.D., Biochemistry; University of Wisconsin, Madison, 1981	Mark Sullivan, Ph.D. Research Associate Professor of Biochemistry & Biophysics in the Center for Oral Biology Primary Appointment: Biochemistry & Biophysics Center Affiliation: Center for Oral Biology
Research: Phage antibody technology in the development of novel reagents for characterization of protein expression and structures See the Sullivan Lab homepage for details.
Contact Information: E-Mail: Mark_Sullivan@urmc.rochester.edu
University of Rochester School of Medicine and Dentistry 601 Elmwood Ave, Box # Rochester, New York 14642	Phone: (585) 273-1629 Fax: (585) 276-0190 KMRB G9627

Research Overview

The ability to rapidly isolate human antibodies using phage display has opened up new approaches to understanding protein function. Research in my laboratory is focused on the manipulation of immunoglobulin molecules to develop novel reagents for characterization of protein expression and structure. We are using phage antibody technology in several areas of proteomics research to develop improved reagents for protein characterization as well as novel inhibitors of protein function that will aid in defining the roles of the many new proteins discovered through genomic sequencing. Major efforts are aimed at dissecting the saliva proteome and developing panels of antibodies for incorporation into new diagnostic platforms using protein microarrays. A second initiative is to derive antibodies useful for solving the structure of proteins by X-ray crystallography. Additional studies focus on the therapeutic potential of single-chain antibodies to reduce protein aggregation and misfolding, a process that characterizes a variety of neurodegenerative diseases.

Publications

Recent Publications:

1.Bliss JM, Sullivan MA, Malone J, Haidaris CG. Differentiation of Candida albicans and Candida dublinensis using recombinant human antibody single chain variable fragments specific for hyphae. J Clin Microbiol 41:1152-1160, 2003

2. Haidaris CG, Malone J, Sherril LA, Bliss JM, Gaspari AA, Insel RA, Sullivan MA. Recombinant human antibody single chain variable fragments reactive with Candida albicans surface antigens. J Immunol Methods 257:185-202, 2001

3. Malone J, Sullivan MA. Analysis of antibody selection by phage display utilizing anti-phenobarbital antibodies. J Mol Recog 9:738-745, 1996

4. Murali R, Helmer-Critterich M, Sharkey DJ, Scalice ER, Daiss JL, Sullivan MA, Krishna Murthy HM. Structural studies on an inhibitory antibody against Thermus aquaticus DNA polymerase suggest mode of inhibition. Protein Eng 11:79-86, 1998

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