Anne Luebke - Current Research
Adenovirus
Adenovirus [E1 - , E3 - ] infects the infused cochlea, and compromises cochlear function. A. Equilevel DPOAE measurements (L 1 =L 2 =70 dB SPL) are shown for the left (perfused) and right (non-perfused) ears of three animals that received [E1 - , E3 - ] adenovirus at 5x10 8 bfu/cochlea. A. Compared to 30 min post-pump implantation (closed symbols), by 8 d post-infusion (open symbols) there was a significant loss in DPOAEs in the infused cochlea. B: In contrast, the non-infused ears, did not exhibit any significant losses in DPOAE levels suggesting that the reduced emissions in the perfused ears were due to a local virally-mediated response. C and D: DPOAE measurements (L 1 =L 2 =70 dB SPL) are shown for the left (perfused) and right (non-perfused) ears of three animals that received [E1 - , E3 - , pol - ] adenovirus at 5x 10 8 bfu/cochlea. C: A modified adenovirus [E1 - , E3 - , pol - ] infected the infused cochlea without compromising cochlear function. Compared to 30 min post-pump implantation (open symbols), there were no significant DPOAE changes by 8 d post-infusion (closed symbols). This finding is in sharp contrast to those with the [E1 - , E3 - ] adenovirus in which the DPOAEs were reduced and remained at noise-floor levels by 8 d post-infusion (panel A). D: As noted above, there was no significant change in the DPOAEs measured in the right (non-perfused) ear.