Basic Sciences Core Director: Sanjay Maggirwar, Ph.D. This Core enhances basic and laboratory-based HIV/AIDS research by providing access to cutting-edge instrumentation and technologies, and the necessary support, education and training to enable efficient use of these resources.The Basic Sciences Core aims to enhance and foster new developments in HIV/AIDS and HIV-related research by broadening research capabilities and promoting collaboration. To enhance the use and utility of cutting-edge instrumentation, CFAR offers funding vouchers for direct application to instrumentation that best suits research needs. Vouchers are competitively awarded and can provide up to $4,000 in user fees. Click here to learn more. Instrumentation and Services Rapid-quench Enzyme Kinetic Analysis Provides access to the Kintek RQF-3 Rapid Quench-Flow instrument to examine fast reaction times (2.5 ms) using extremely small sample volumes, and allowing double delay quench which is highly suitable for examination of enzyme reaction pathways. http://www.kintek-corp.com/index.phpLearn more. Next Generation Sequencing (NGS) Consultation services are available to assist faculty with performing transcriptional profiling or studies of viral mutagenesis and use of the new Illumina Hi-Seq 2500 high throughput sequencer within the Functional Genomic Center at the URMC. Illumina Hi-Seq 2500 - consultation services available to assist faculty with performing transcriptional profiling or studies of viral mutagenesis. The MiSeq desktop sequencer allows you to access more focused applications such as targeted gene sequencing, metagenomics, small genome sequencing, targeted gene expression, amplicon sequencing, and HLA typing. Structural Biology Provides users access to biophysical instrumentation designed to: (i) determine macromolecular structures by X-ray crystallography, and (ii) quantitatively characterize biomolecular interactions. This facility provides CFAR supported technical assistance to provide Investigators with theoretical input, practical training and experimental design and data interpretation. Instruments include: BIAcore T100, Bruker AXS X8 Prospector X-ray source, Mosquito robot . For information and support, please contact Jermaine Jenkins. AKTA Pure Chromatography System - A pure chromatography system for purification of RNA, proteins, peptides or complexes thereof. Researchers working on HIV/AIDS or related areas are eligible for discounts (50% off whole day and 25% off a 4 hour block) View Flyer Imaging and Mass Spectrometry Provides state-of-the-art instrumentation along with the human expertise to support all that is possible now, while pushing the limits of what can be done with flow cytometry. These next-generation instruments provide a host of new applications not readily available using traditional flow cytometry or mass spectrometry. Amnis ImageStream Gen-X CyTOF Instrument High Throughput Screening Provides low cost access to screening chemical libraries for identification of novel molecules that can be used to perturb biological systems. This center develops assays for high throughput screening, and has the ability to screen relatively small libraries to obtain preliminary data for grant proposals as well as to screen much larger libraries for identification of leads for therapeutics. Customized Protein Production Specialized protein production service that produces, purifies and characterizes HIV-1 Env oligomers for HIV/AIDS research. This includes: Recombinant oligomeric HIV-1 gp140 (produced in human cells) Monoclonal Antibodies (produced by transient DNA transfection of 293 cells) His-tagged recombinant RT (produced in E. coli) Custom proteins of interest, produced as His-tagged moleculed in E. coli For information about protein production, please contact Jennifer Lynch.