10:00am - 11:30am|
CRISPR-Cas: Tools and applications for mammalian genome engineering
Presented by: Fei Ann Ran, PhD
Post-Doctoral Fellow in the Feng Zhang Lab;
Junior Fellow, Harvard Society of Fellows;
Eli and Edythe L. Broad Institute of Harvard and MIT
The ability to introduce targeted modifications into genomes and engineer model organisms holds enormous promise for biomedical and technological applications, and has driven the development of tools such as zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs). We have harnessed the Cas9 nuclease from the bacterial CRISPR (clustered regularly interspaced short palindromic repeats) adaptive immune system for targeted editing of the mammalian genome. Cas9 can be easily programmed by short guide RNAs to induce gene knockout or homology-directed repair with robust efficiency and is highly amenable to multiplexed applications. In addition, we have also engineered a Cas9 nicking mutant and devised a "double nicking" strategy to facilitate high-precision genome editing with minimal off-target mutagenic activity, enabling applications across basic science, biotechnology, and medicine. The seminar will also discuss the newest developments in using Cas9 for in vivo and therapeutic applications.
Posted by: Anne Reed, Shared Resource Laboratories, 24-Jul-14 8:01am ET