Welcome to the Halterman Lab

Molecular-Based Therapies for Ischemic Brain Injury

We are interested in finding new approaches to treat ischemic brain injury. In particular, our lab focuses on signaling nodes in transcriptional networks that regulate neuron survival and are amenable to small molecule regulation. Putative targets identified through genomic and proteomic-based screens are first validated in vivo and studied further using primary neuronal cultures, neurospheres and related in vitro assays. To manipulate gene function we use several complementary approaches including knockout mouse models, viral mediated gene regulation, site-directed mutagenesis and pharmacological inhibition. Ultimately, our goal is to identify novel therapeutic targets and treatments for disorders in which ischemia is a central component.

Fig 1A

Control (left) and ischemia-induced nuclear condensation (right) in neurons of the CA1 field of the hippocampus following 25 minutes bilateral carotid occlusion. Sections were stained with Hoechst and imaged using optical sectioning microscopy. Nuclear area was measured directly in ImageJ.

Sham and BCCAO

Fig 1B

Corresponding frequency distributions of nuclear area (µm2). Normal curves were fit through method of least squares, constrained by a SD of 15 or less.

Sham 25-min Inj

Fig 2

Doublestaining of the LysGFP knock in mouse cortex. PECAM blood vessels(red), Laminin, basal lamina(blue) Neutrophils(green). Notice the perivascular machrophages that line the small blood vessels in brain. Magn. 40x

a

Recent publications

Showing 2 of 33 journal articles.

Matthews TA; Abel A; Demme C; Sherman T; Pan PW; Halterman MW; Parkkila S; Nehrke K. "Expression of the CHOP-inducible carbonic anhydrase CAVI-b is required for BDNF-mediated protection from hypoxia." Brain research. 2014; 1543():28-37. Epub 2013 Nov 23.

Rininger A; Wayland A; Prifti V; Halterman MW. "Assessment of CA1 injury after global ischemia using supervised 2D analyses of nuclear pyknosis." Journal of neuroscience methods.. 2012; 207(2):181-8. Epub 2012 Apr 21.

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