Joseph M. Miano, Ph.D.
Associate Director - Aab Cardiovascular Research Institute
|Professor - Department of Medicine, Aab Cardiovascular Research Institute|
|1992 | Ph.D. | Experimental Pathology | New York Medical College|
|1988 | M.S. | Experimental Pathology | New York Medical College|
|1986 | B.S. | Biology | SUNY College at Cortland|
Altered programs of cellular differentiation underlie most complex diseases. For example, smooth muscle cells (SMC) of the blood vessel wall undergo de-differentiation in various human diseases such as atherosclerosis and transplant arteriopathy. The Miano Lab utilizes tools in bioinformatics and genomics to interrogate vascular disease models (A) and cultured human coronary SMC (B) to discover novel DNA elements and genes in human/mouse genomes that impinge on the SMC differentiation program. Current funded projects include the definition of the CArGome, which is the full complement of serum response factor (SRF) binding CArG elements in human/mouse genomes, and CArG-SNPs (CArG Variome) that disrupt normal SRF binding (C); the discovery and functional analysis of new SRF/Myocardin (SRF cofactor) target genes such as AKAP12A and LMOD1 (D); and the discovery of long noncoding RNA (lncRNA) genes, such as the recently defined SENCR gene (E). LncRNA genes, which already out number all protein-coding genes and are emerging as critical mediators of cellular homeostasis, are the subject of intense study in the lab using various molecular (eg, Northern/RACE/qRT-PCR/luciferase), cell biological (RNA-FISH), biochemical (RNA pulldown), and genomic (RNA-seq/RIP-seq) assays (F). This discovery-based research culminates with in vivo functionality of CArG elements (or CArG-SNPs), protein-coding genes (AKAP12A/LMOD1) as well as lncRNA genes (G) using the revolutionary CRISPR/Cas9 genome editing system in mice. In this manner, the lab is able to rapidly (few months) assess the role of CArG boxes or various genes in an in vivo setting and in doing so develop new ways of understanding the pathogenesis of human diseases (H).
The Miano Lab’s ideas and efforts span the spectrum from computer to DNA to cells to whole animals. The lab intends to elucidate the regulation of priority genes and their functions during normal or pathological processes involving, but not limited to, the cardiovascular system. Work in the Miano Lab is necessarily multi-disciplinary and therefore provides ample opportunities for trainees to learn and utilize state-of-the-art technologies in genomics, genetics, bioinformatics, vascular pathobiology, and gene transcription control.
Miano Lab Research Pipeline (2014)
Miano's Suggested Links
Joseph Miano , PhD
University of Rochester
School of Medicine and Dentistry
601 Elmwood Ave, Box CVRI
Rochester, New York 14642
Miano Lab: (585) 276-7725