Color High-Resolution Plastic Sections

Color High-Resolution (2.5 micron) Plastic Sections Utilizing Basic Fuchsin and Methylene Blue as a Hematoxylin & Eosin (H & E) Like Stain and Trichrome-Like Stain

This colorful plastic section technique allows the investigator to examine large tissue blocks (1.0 x 1.0 x 0.2 cm) which are processed and embedded in Spurr epoxy resin for 2.5 um plastic sectioning using a JB-4 microtome. The sections are attached to glass slides and stained sequentially with methylene blue and basic fuchsin and coverslipped. The result is a brilliant H & E-like staining of the nuclear and cytoplasmic elements with a Trichrome-like stain. Listed below are the various cellular components in tissues that can be identified by this unique plastic section staining technique. Specific areas or cells can also be "popped off" these stained sections for ultrastructural examination.

Cellular Component Stain Color . Cellular Component Stain Color
collagen turquoise blue elastin brilliant red-purple
glycogen blue lipid olive green
myelin dark purple intestinal mucin pink to purple
gastric mucin gray purple mast cell granules purple
lipofucsin green-black melanin mahogany
basement membrane pink    
Color 1 Color 2
Color 3 Color 4

Ref: di Sant'Agnese, P.A. and de Mesy Jensen (Bentley), K.L.: Dibasic staining of large epoxy tissue sections and applications to surgical pathology. Amer. J. Clin. Pathol. 81:25, 1984.

Contact Us

Karen de Mesy Bentley, M.S., Director
Phone: (585) 275-1954
Office: B-7806
EM Labs: B-7811, B-7819