Optical Micro-Machining using Femtosecond Lasers Project Overview IRIS stands for Intratissue Refractive Index Shaping. It is a process invented at the University of Rochester by a team of scientists under the leadership of Dr's. Krystel Huxlin and Wayne Knox who demonstrated that it was possible to use a low-energy, high-repetition rate femtosecond laser to alter the refractive index of transparent, living tissues (such as the cornea and lens) without causing massive amounts of tissue damage or inducing a wound healing and scarring response. Our long-term goal with this research is to develop the use of femtosecond micromachining as a non-damaging method of customizing the refractive correction in a human eye, be it in the cornea, lens or implanted IOLs. Current experiments are first testing and developing this technology in animal models, with specific projects including: assessing the durability of inscribed femto-IRIS patterns in a living cornea over time, assessing the biological reaction and especially, the presence or absence of a corneal wound healing reaction to an inscribed femto-IRIS pattern in a living cornea over time, assessing the wavefront aberration changes induced by a spherical or cylindrical IRIS pattern over time.