Facilities & Resources
The FGC provides core laboratory support, technical advice, and assistance with experimental design and data analysis for UR investigators in DNA sequencing, genotyping, gene expression, and RNA and DNA processing (extraction, purification, quantitation). It has a faculty-level director (Stephen Welle, Ph.D., Professor of Medicine), 5 technical staff members (including a data analyst), and a computer systems manager.
The FGC is located at the UR Cardiovascular Research Institute, 5 mi. from the university medical center (URMC). At the URMC, there is a sample drop off location with -80 freezer and refrigerator, and twice daily courier service to deliver samples from URMC to the FGC lab. This FGC has approximately 2500 sq. ft. of laboratory space and 1800 sq. ft. of office space. The FGC also has a meeting room with computer at the medical center, and the FGC director has a second office located at the URMC.
The FGC has 14 desk-top computers and 3 quad processor servers linked to the internet and to the UR intranet, and a computer cluster with 6 nodes (24 GB each) and 16 TB disk storage for analysis of high-throughput sequencing data. A LIMS program is used for web-based sample submission and delivery of results files. The FGC has its own systems administrator who is responsible for maintaining this infrastructure, and a data analyst to assist investigators with microarray, real time PCR, and high-throughput sequencing data sets.
The following major equipment is located in the FGC: SOLiD 4 high throughput sequencer (Applied Biosystems), Illumina GAIIx high throughput sequencer, Affymetrix GeneChip System (Hybridization Oven 640, Fluidics Station 450, Scanner 3000), Perkin Elmer ScanArray Microarray Scanner, ABI 7900HT real-time PCR instrument with autoloader and microfluidic card module, ABI 3730 Capillary Electrophoresis DNA sequencer, 25 Thermo Hybaid MBS PCR machines, CAS 1200 liquid handling robot, Agilent 2100 Bioanalyzer, BioRad gel documentation system, 3 NanoDrop ND-1000 spectrophotometers (two at FGC, one at medical center for public use), SciGene hybridization oven, Tecan HS4800 hybridization station.
Download - Word document's listing FGC resources.
