Next Generation Sequencing (NGS)

 

At the URGRC, we utilize Illumina's sequencing by synthesis (SBS) technology.  Illumina is currently the most successful and widely used NGS platform.

Instrumentation

Illumina HiSeq2500 Sequencer

  • 600 GB data output
  • On board pair end turnaround
  • High output (2 flow cells)
  • 180 - 360 million reads per lane of flow cell (single vs. pair end)
    • 6 billion reads per full run of 2 flow cells
  • Current read lengths:
    • 1x100, 1x150 (Single End Read)
      • ~7 day run
    • 2x100, 2x150 (Pair End Read)
      • ~12 day run

cBot

  • Cluster amplification of sample library

 

Applications

  • Transcriptomics
    • RNA Sequencing
          • Standard 100ng input
            • Poly A capture (mRNA-seq)
            • Ribosomal RNA (rRNA) depletion (recommend for prokaryotic samples and for identification of long non-coding RNA)
          • Low input mRNA-seq protocols (~1ng)
          • Single cell sequencing
    • Small RNA Sequencing
  • Gene Regulation and Epigenomics
      • ChIP Sequencing
      • Bisulphite Sequencing
      • MeDIP Sequencing
  • Genomics
    • Whole Genome Sequencing
    • Exome Sequencing
  • Contact us for custom applications.

For Illumina NGS library preparation we utilize kit based workflows supplied by:

Data Analysis

The URGRC currently has a bioinformatician on staff with experience in NGS data analysis methods and workflows.  For more information please contact us.