Sample Submission Guidelines For NGS

Transcriptomics

RNA Sequencing

If providing tissue or cells, we require enough material to obtain at least 0.2 - 0.5ug of total RNA.  For purified RNA, we recommed submiting at least 0.2ug of purified total RNA in ultra pure water. DNase treatment of RNA is necessary and can be done prior to submission using Turbo DNase or the GRC will perform this for a fee.   Sample quantity and quality will be verified with spectrophotometry (NanoDrop 1000), fluorometry (Qubit), and the Agilent Bioanalyzer 2100 profile.  RIN (RNA Integrity Number) values of >7.0 and OD260/280 = 2.0 - 2..2 are recommended for RNA-seq library preparation.  For samples with low RNA abundance, there are alterantive methods that can be used for RNA inputs of 1ng and below.  Contact us for additonal information on low input RNA-seq.  Please fill out the RNA Sample submission form in its entirety and place your samples in -80C located in the URGRC Drop-off room on the Medical Campus.

Small RNA Sequencing

If providing tissue or cells, we require enough material to obtain at least 2-5ug of total RNA.  For purified RNA, we recommed submiting at least 2-5ug of purified total RNA or enriched small RNA from 2-5ug total RNA (~50ng) in ultra pure water. DNase treatment of RNA is necessary and can be done prior to submission using Turbo DNase or the GRC will perform this for a fee.   Sample quantity and quality will be verified with spectrophotometry (NanoDrop 1000), fluorometry (Qubit), and the Agilent Bioanalyzer 2100 profile.   Please fill out the RNA Sample submission form in its entirety and place your samples in -80C located in the URGRC Drop-off room on the Medical Campus.

 

Genomics

Whole Genome Resequencing

If providing tissue, blood or cells, we require enough material to obtain at least 2ug of total gDNA.  For purified gDNA, we recommed submiting at least 2ug of purified total gDNA in ultra pure water.  Sample quantity and quality will be verified with spectrophotometry (NanoDrop 1000), fluorometry (Qubit), and gel electropheresis.  We recommend DNA purity with an OD260/280=1.8-2.0 without degradation or RNA contamination.    Please fill out the DNA Sample submission form in its entirety and place your samples in -80C located in the URGRC Drop-off room on the Medical Campus.

Exome Sequencing

Methods currently under evaluation ...

 

Epigenomics

ChIP Sequencing

Please submit at least 20-50ng of ChIP or capture kit enriched DNA in ultra pure water.  We strongly recommend verification of the ChIP samples with qPCR prior to submission for ChIP-seq library contruction.  Sample quantity and quality will be verified with spectrophotometry (NanoDrop 1000), fluorometry (Qubit), and the Agilent Bioanalyzer 2100 profile.  We recommend DNA purity with an OD260/280=1.8-2.0 without RNA contamination.  Please fill out the DNA Sample submission form in its entirety and place your samples in -80C located in the URGRC Drop-off room on the Medical Campus.

Bisulphite Sequencing

Methods currently under evaluation ...

 

MeDIP Sequencing

Methods currently under evaluation ...