Nucleic Acid Quality Assessment

NanoDrop® Spectrophotometer

Instrument: NanoDrop® ND-1000 Spectrophotometer
Application: Accurate and reproducible measurement of nucleic acid samples; use only 1-2 uL without dilution.

This is a free service provided by the URGRC; NanoDrop® is available for your use in the Medical Center, room 3-6807.

Note: The NanoDrop® is unavailable on Thursday mornings from 8:45 to 10:45 and network connectivity is not working correctly. Please remember to bring either a diskette or USB memory stick for your data.

Please see the user manual for more detailed information on the instrument's use.

Key Features

  • Full spectrum data is automatically archived in a text delimited file on the controller PC and is easily reviewed using the Data Viewer feature.
  • 260/280 and 260/230 ratios calculated and displayed.
    • DNA Quality:
      • DNA should be quantified using a spectrophotometer. We have provided a Nanodrop workstation for free general use in G7415 at the Medical Center. As a general guideline, DNA free of major contamination should provide OD260/280 of 1.7 - 1.9 and OD260/230 of ~2.0. Lower OD260/280 usually indicates protein contamination while high ratios are indicative of organic contamination.

      • The URGRC also has a Qubit  Flourometer for nucleic acid quantitation.

      • Comparison of Qubit   vs. Nanodrop 1000.

 

Sample Output Measurement

NanoDrop sample output measurement

 

 

 

Agilent 2100 Bioanalyzer

Instrument: Agilent 2100 bioanalyzer
Application: Faster than gels (digital data in 30 minutes for quality and quantity of up to 11 samples); reproducible and complete digital data.

RNA samples can be submitted to the URGRC for analysis:

Links for Additional Information

Sample Output Measurement

Agilent 2100 bioanalyzer sample output measurement

Agilent 2100 bioanalyzer sample output measurement

 

Questions?

For questions regarding these services, please contact us.