Affymetrix Genechip® 3' Expression Arrays
General Information
- Instrumentation: GeneChip® Fluidics Station 450, GeneChip® Scanner 3000 7G.
- The FGC can process most 3' expression arrays that Affymetrix offers.
- We stock Human U133 Plus 2.0, Mouse M430 2.0, and Rat 230 2.0 arrays. All other arrays must be purchased as an entire pack. Affymetrix Chip Pricing
- Processing protocols: Nugen-Ovation or Ambion MessageAmp
- $400 Lab processing fee includes: RNA quality check on the Agilent Bioanalyzer, target preparation and labeling, sample hybridization, array scanning, and data QC.
Optional Sample Preparation Services*
- RNA Extractions
- RNA quality check on the Agilent Bioanalyzer**
- Real-time PCR amplification for array validation
**However, if the RNA sample is submitted for microarray analysis, there will be no charge for the bioanalyzer service. See our Pricing page for complete information.
Sample Submission
- We recommend for RNA isolation: Trizol (Invitrogen) extraction followed by the RNeasy Clean-up protocol with a DNase treatment (Qiagen).
- Please submit at least 2 ug total RNA in a concentration of 100-200 ng/ul if possible.
- When necessary, we can perform analysis on less than100ng total RNA; please contact us for more information.
- Complete Microarray Expression Request Form, place samples and form in a Ziploc bag and drop-off in -80C Freezer (Medical Center Ground Floor, room G-7814).
Data Retrieval and Analysis
- We have a remotely accessible workstation. Contact FGC for more information.
- Contact FGC by phone at (585) 276-9997 or by email for more information and any questions you may have.
More Information
For further information, please contact Michelle Zanche, (585) 276-9997.





