Conc

Submitting Your Samples

 

The URGRC offers 4 options for DNA sequencing sample submission:

 

Standard (Preferred)

  • You supply the DNA template for sequencing and we perform the reaction using our standard primers.    If you supply custom primers for sequencing, please submit them using the "Pre-mixed" option below.
    • DNA should be diluted with sterile RNase/DNase-free water.
    • 0.6ml or 1.5ml eppendorf tubes are preferred but we will accept strip cap PCR tubes if properly labeled.  Please do not submit multiple 0.2ml PCR tubes loosly in a ziploc bag.
DNA Template
Size
Quantity
Volume
Concentration
Plasmid DNA <10kb 400ng 10ul
40ng/ul
Plasmid DNA >10kb 2000ng 20ul
100ng/ul
PCR Product 100-200bp 20ng 10ul
2ng/ul
PCR Product 200-500bp 40ng 10ul
4ng/ul
PCR Product 500-1000bp 80ng 10ul
8ng/ul
PCR Product 1000-2000bp 100ng 10ul
10ng/ul
PCR Product >2kb 400ng 10ul
40ng/ul

**We ask that you please send in the volumes stated above in order to have enough material to repeat reactions if necessary.**

 

Pre-mixed (Preferred)

  • You supply the DNA template and primer already mixed together.
    • DNA/primer mix should be diluted with sterile RNase/DNase-free water.
    • 0.6ml or 1.5ml eppendorf tubes are preferred but we will accept strip cap PCR tubes if properly labeled.  Please do not submit multiple 0.2ml PCR tubes loosly in a ziploc bag.
DNA Template
Size
Quantity

Primer Amount

(pmol)

Volume
Plasmid DNA <10kb ~400ng 20pmol 12ul
Plasmid DNA >10kb ~2000ng 40pmol 24ul
PCR Product 100-200bp ~20ng 20pmol 12ul
PCR Product 200-500bp ~40ng 20pmol 12ul
PCR Product 500-1000bp ~100ng 20pmol 12ul
PCR Product 1000-2000bp ~100ng 20pmol 12ul
PCR Product >2kb ~400ng 20pmol 12ul

**We ask that you please send in the volumes stated above in order to have enough material to repeat reactions if necessary.**

 

Seq Drop Off

  • A.) You perform the BigDye labeling reaction in your lab and send the sample to us for post-reaction cleanup.  BigDye can be ordered through the URGRC by following this link
  • B.) You can also perform the post reaction cleanup in your lab using Princeton Separations Centri-Sep spin columns or Abgene Dye Terminator Removal Plates. The samples should then be dried down in 1.5ml eppendorf tube and sent to us for sequencing.

**Please be sure to select YES for option A and NO for option B in the"Sequencing Reaction Clean-up" field when you enter the sample information on dnaLIMS.**

 

dnaLIMS

NanoDrop Spectrophotometer

The NanoDrop is a free service offered by URGRC and is located in the Medical Center, room G-7415. Please see the user manual for more detailed information.

 

Questions?

For questions or feedback regarding DNA Sequencing services, please contact us.