Next Generation Sequencing (HiSeq2500)
*All pricing effective February 2013 and is subject to change.
**Prices reflective of University of Rochester internal usage, external pricing is available. Contact us for more information.
Library Preparation
Library preparation |
Cost per sample | High Throughput rate per sample |
|---|---|---|
| mRNA-seq | $179.00 | n/a |
|
(Human, Mouse, Rat) |
$244.00 | n/a |
| Small RNA-seq | $205.00 | n/a |
|
(Clontech SMARTer Technology) |
$265.00* | $211.00* |
| DNA-seq | $179.00 | n/a |
| ChIP-seq | $340.00 | n/a |
| Large insert (Gel-Free) | $181.00 | n/a |
Large insert (Gel-Plus) |
$454.00 | n/a |
| Mate Pair (2-5kb) | $501.00 | n/a |
| Nextera XT | $60.00 | n/a |
| Whole Genome Bisulphite (WGBS) | $180.00 | n/a |
| PCR Free DNA Preparation | $188.00 | n/a |
* Includes library preparation.
Sequencing
High Output Run (~4-6 week turnaround)*
Single End Read (SER)
| SER | Cost per lane |
|---|---|
| 1 x 50 | $1037.00 |
| 1 x 100 | $1371.00 |
Pair End Read (PER)
| PER | Cost per lane |
|---|---|
| 2 x 50 | $1680.00 |
| 2 x 100 | $2223.00 |
Rapid Run (~2-4 week turnaround)*
Single End read (SER)
| SER | Cost per lane |
|---|---|
| 1 x 50 | $1359.00 |
| 1 x 100 | $1859.00 |
| 1 x 150 | $2359.00 |
Pair End Read (PER)
| PER | Cost per lane |
|---|---|
| 2 x 50 | $1423.00 |
| 2 x 100 | $3148.00 |
| 2 x 150 | $4148.00 |
Contact us for information regarding run option for your given application
* Turnaround time depends on sample queue at time of submission and is subject to change based on usage.
** Sequencing costs include basic data analysis:
- Demultiplexing
- Read processing (filtering based on complexity and end trimming based on quality scores)
- Mapping to reference genome
Additional analysis support can be provided. Contact us for more information.
Bioanalyzer Analysis
| Sample Type | Chip | Amount Required | Cost |
|---|---|---|---|
| RNA | Nano | 25 - 500ng/ul | $7.00 |
| RNA | Pico | 0.05 - 25ng/ul | $7.00 |
Small RNA (6-150bp) |
Small RNA | 1 - 100ng/ul (total RNA)l or 1 - 20ng/ul (enriched for small RNA) | $30.00 per chip* and additional $3.00 for sample processing |
DNA (50bp - 7kb) |
High Sensitivity | 5 - 500pg/ul | $40.00 per chip* and additional $3.00 for sample processing |
DNA (25bp - 1kb) |
DNA1000 | 0.5 - 50ng/ul | $7.50 |
DNA (100bp - 12kb) |
DNA12000 | 0.5 - 50ng/ul | $7.50 |
* 10 samples per chip.
RNA Extraction/Purification
Trizol RNA Extraction - $25.00/sample*
Qiagen RNeasy Plus - $25.00/sample*
Dnase Treatment - $15.00/sample
* Includes quantification and quality assessment
DNA Extraction/Purification
Qiagen DNeasy Kit - $25.00/sample*
* Includes quantification and quality assessment
Single Pass DNA Primer Extension (Sanger DNA Sequencing)
Standard Reaction - $5.50/ reaction ($450.00 per 96 well plate)
Pre-Mixed Reaction - $5.50/ reaction ($450.00 per 96 well plate)
Seq Drop Off:
- Sequencing only - $3.50/ sample ($120.00 per 96 well plate)
- Cleanup and sequencing - $4.50/ sample ($220.00 per 96 well plate)
Difficult Template (GC-rich, Hairpin, etc..) - $6.50/ sample
Quantitative PCR
| Service | Cost | Quantity |
| ABI High Capacity Reverse Transcription | $8.50 | Sample |
| Nugen WT-Ovation PicoSL Kit (RNA Amplification) | $60.00 | Sample |
| qPCR Reaction Set-up (Does not include Primers And Probe) | $2.00 | Well |
| qPCR Reaction Set-up (Using FGC Endogenous Control Assay) | $2.50 | Well |
| 7900 Instrument Run Fee | $60.00 | Plate |
| ABI Microfluidic Card (TLDA) Set-up And Run On 7900 (Does not include TLDA) | $80.00 | TLDA |
Micro Array Analysis
| Service | Cost | Quantity |
| Processing Fee, preparation with NuGEN (<100ng) | $400.00 | Sample + $250 project fee |
| Processing Fee, preparation with Ambion (>100ng) | $360.00 | Sample + $250 project fee |
| Affymetrix 3' IVT Genechips, most common species | $400.00 | Array |
| Affymetrix Gene 2.0 ST Genechips, most common species | $175.00 | Array |
Genotyping
| Service | Cost | Quantity |
| PCR amplification (Does not include primers) | $3.00 | Sample |
| Gel Electrophoresis | $3.00 | Sample |
| PCR purification | $3.00 | Sample |
| 3730 Fragment Analysis, FGC Adds Formamide & LIZ-500 | $1.00 | Sample + $25.00 Setup Fee |
| 3730 Fragment Analysis, FGC Adds Formamide & LIZ-500 | $100.0 | 96-well Plate, Max Charge |
| ***High-throughput discount if 8 or more plates are submitted per order - $85/plate | $85.00 | Plate |
| 3730 Fragment Analysis Ready to Run, Customer added Formamide & LIZ500 standard | $70.00 | 96-well Plate, Max Charge |
