Real Time PCR—Instrument Run Only

General Information

  • Instrumentation: Applied Biosystems 7900HT Sequence Detection System
  • Applications: Absolute Quantification, Relative Gene Expression, and Allelic Discrimination for SNP genotyping
  • Available Formats: 384-well plate (no 96-well plates)
  • Customer Provides: Prepared 384-well reaction plate and Real Time PCR LIMS request form
  • Service Includes: Instrument run and data upload to LIMS

Optional Assay Order Services*

  • Primers and probes can be special ordered through Applied Biosystems (ABI)
  • Order requests can be placed through Michelle Zanche.

Optional Sample Preparation Services*

  • RNA extractions (includes Bioanalyzer analysis)
  • Bioanalyzer analysis of customer prepared RNA
  • cDNA synthesis (requires previous Bioanalyzer analysis)
  • RNA amplification (NuGEN WT-Ovation)
  • Real Time PCR assay optimization and validation

Sample Submission

  • If you require sample preparation services, please submit appropriate form.
  • Reserve a run time on the 7900HT.
  • Create a Real Time LIMS request form.
  • Click for instructions on using Real Time PCR LIMS for submitting your prepared reaction plate
  • If using SYBR green, please submit a melting curve file in addition to your .sds run file (link to SYBR rq melting curve).
  • Place your 384-well reaction plate and LIMS form in a Ziploc bag and submit to the 4°C refrigerator (MED G-7814).

Data Retrieval and Analysis

*Note: The optional services are not included in the Real Time PCR pricing. See our Pricing page for complete information.

For questions regarding Real Time PCR services, please contact Michelle Zanche, (585) 276-9997.