Real Time PCR—Instrument Run Only
- Instrumentation: Applied Biosystems 7900HT Sequence Detection System
- Applications: Absolute Quantification, Relative Gene Expression, and Allelic Discrimination for SNP genotyping
- Available Formats: 384-well plate (no 96-well plates)
- Customer Provides: Prepared 384-well reaction plate and Real Time PCR LIMS request form
- Service Includes: Instrument run and data upload to LIMS
Optional Assay Order Services*
- Primers and probes can be special ordered through Applied Biosystems (ABI)
- Order requests can be placed through Michelle Zanche.
Optional Sample Preparation Services*
- RNA extractions (includes Bioanalyzer analysis)
- Bioanalyzer analysis of customer prepared RNA
- cDNA synthesis (requires previous Bioanalyzer analysis)
- RNA amplification (NuGEN WT-Ovation)
- Real Time PCR assay optimization and validation
- If you require sample preparation services, please submit appropriate form.
- Create a Real Time LIMS request form.
- Click for instructions on using Real Time PCR LIMS for submitting your prepared reaction plate
- If using SYBR green, please submit a melting curve file in addition to your .sds run file (link to SYBR rq melting curve).
- Place your 384-well reaction plate and LIMS form in a Ziploc bag and submit to the 4°C refrigerator (MED G-7814).
Data Retrieval and Analysis
*Note: The optional services are not included in the Real Time PCR pricing. See our Pricing page for complete information.
For questions regarding Real Time PCR services, please contact us.