Real-Time PCR

Setup and Instrument Run by the URGRC

General Information

  • Instrumentation: Applied BiosystemsQuantStudio 12K Flex Real-time PCR system; Corbett Robotics CAS-1200 precision liquid handling system for plate set-up.
  • Applications: Absolute Quantification, Relative Gene Expression, Allelic Discrimination for SNP genotyping, digital PCR
  • Available Formats: 96-well, 384-well plate, ABI Taqman Low Density Array (microfluidic card), and OpenArray (Digital).
  • Customer Provides: Probes and primers
  • Service Includes: Setup of real time PCR reactions (includes "no-RT" control) using ABI Taqman or SYBR Green master mix and instrument run

Optional Sample Preparation Services*

  • RNA extractions (includes Bioanalyzer analysis)
  • Bioanalyzer analysis of customer prepared RNA
  • cDNA synthesis (requires previous Bioanalyzer analysis)
  • RNA amplification (NuGEN WT-Ovation)
  • Real Time PCR assay optimization and validation

 

Sample Submission

  • Please submit appropriate form with your samples.

Data Retrieval and Analysis

*Note: The optional services are not included in the Real Time PCR pricing. See our Pricing page for complete information.

For questions regarding Real Time PCR services, please contact us.