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This page will house old news and updates pertaining to the Flow Core here at the University of Rochester.
Archives - 2009
21 September 2009
Greetings all! We would like to inform all users that the 11 color LSRII in the RHIC is now a 12-color LSRII. A third PMT has been added to the violet laser. Please see the Analysis page for new voltages and filter configurations.
3 September 2009
Our newest instrument, the Amnis ImageStream GenX, is here and open for use! The relevant information is under Instruments > Imaging. Also, do not hesitate to ask Tim or Dave if you have any questions.
28 August 2009
The new Wednesday lecture series kicks off starting the 9th of September. See the right side for topics, location, and time. Also, the Accuri C6 cytometer is here! Please contact Tim if you would like more information about this instrument.
3 August 2009
The slides for the Introduction to Flow Lecture can be found on the Training page or by following this link.
29 June 2009
The FACSAria cell sorter has been moved to it's new home in the 3-4100 area! It is available for use again starting today.
27 May 2009
The sort form and guidelines for nozzle switching have been changed. Please be sure to familiarize yourself with the new information and the new sort form. As always, please let us know if you have any problems.
16 April 2009
The linear range of all PMTs and the suggested voltage is now listed for each instrument available in the Flow Core and RHIC. Please see the Analysis page for the values on each instrument.
14 April 2009
Oh boy, pictures! Tim's lecture for Thursday is now posted for your downloading enjoyment.
9 April 2009
The new fluorchrome naming policy was rolled out this past Monday. Please see the link for the new list in the library and of course do not hesitate to see any staff if you have a problem.
3 April 2009
The lecture for this Thursday's installment of Tim's lecture series is now available on the Lectures page!
1 April 2009
Tim's lecture for tomorrow is posted on the Lectures page. Have a look!
Also: There is now a Training link! This is the resource we will use from now on for all training information.
25 March 2009
The lecture for tomorrow has been posted. Grab it from the lectures page!
18 March 2009
The lecture for tomorrow's presentation by Tim has been posted on the lectures page. Get it while it's hot!
Addendum: I am sorry if anyone has tried getting the .pdf of the lecture for tomorrow, but the link was inadvertently broken most of the afternoon. It is now fixed. Thank you.
10 Febuary 2009
Dates for the lecture series have been posted. See the Lectures page for all the dates to save.
5 Febuary 2009
Lectures will now be posted to the Lectures link located in the navigation pane to the left of the page. Upcoming lectures will be announced here, as well as a backlog of all previous lectures, each available for download. Check back often as updates become available!
14 January 2009
The forms should once again be behind the firewall and working. We apologize for the downtime with the forms, but everything should be back to normal once again.
8 January 2009
As a follow-up to the UR Cytometry email sent by Tim, it should be noted that attendance of at least one of the listed lectures is mandatory for all users! If you do not attend, your account will be suspended. Please make sure you can come to one of these 30 minute lectures. Thank you.
5 January 2009
As a note to all users, the sort form, new user form, and cancellation form have been moved behind the firewall to prevent misuse. You will now need to have access to the Intranet by either being on a secure connection at the University or by using the VPN client to connect securely from a remote location. Thank you.
UPDATE: There is currently a problem with submission of the forms. If this happens, please let us know when you tried to submit the form and what browser you used during the process.
Archives - 2008
29 December 2008 - A message from Tim:
I am pleased to announce that the move from MBX 1-11314 to 3-4100 has gone smoothly. The 12-color LSR-II, FACSCanto, FACSCalibur and Imagestream all survived the trip and appear to be working well. We are in the process of validating and characterizing the instruments after their move and realignments.
Starting this week, we will have limited hours in the lab, based on staffing and such.
One important issue that is being addressed now is access to the 3-4100 area. At present and through at least 1/8/2009, the flow core will operate under reduced hours (9 am to 5:30 pm). The reason for this is that we do not have card-swipe access to our facility yet, and do not have sufficient keys to provide individual labs access. We are working with the keyshop to resolve this issue and hope to have card-swipe access on the main door and two analytical rooms (3-4151 and 3-4141) within 2 weeks.
Cell sorting will still be occurring in MRBX 1-11314, and we are working with Janet Ives to develop the new protocols that will be in place to comply with the IBC guidelines. Once those are finalized, I will notify the community as to them.
As always, if you have any questions or comments, please don’t hesitate to contact me directly.
Regards,
Attention Flow Core users:
The core will be shutting down to move on December 22nd. ALL of the instruments in MRBX 1-11314 will be offline for that week. If there are any concerns, please talk to Tim sooner than later so that no problems that arise.
As a note to all Core and HIC user: There is now a reservation system for the HTS units that work in combination with any of the LSRIIs. These can be reserved in the same manner as an instrument found on the online calendar.
An update on the location of the Flow Core:
Renovations to the 3-4100 area are underway as of September 22nd. More information about a possible moving day will be posted as the time draws closer.
A note to users of the FACSVantage instruments:
These will be going offline as of August 1, 2008. Please make sure to familiarize yourself with the FACSAria and Diva software utilized by the Flow Core. It is also recommended that you validate any protocols on the FACSAria before experimentation is done. Please contact Tim if you have any further questions.
Additional information about the billing system in use has been added to the Billing link on the left panel. Please review the new information and speak with Tim if there are any questions or concerns.
The 12-color LSRII in the Flow Lab is now online! The laser configuration is the same as the 11-color LSRII in the HIC with the exception of the Violet laser. Head over to the Analysis page to find information about this difference. Reservations can be made here as well.
The new FACSCanto is on-line and available for use. This is an 8-color insturment running DIVA 6.1. You will need a log-in account. If you do not have one, you will need to apply for one here.
Our new calendar is on-line. Please bookmark http://coreserver.urmc-sh.rochester.edu/cgi-bin/flowcore/flowcore.pl. You will need a log-in to make reservations. You can apply for one here.
We will be bringing on-line a new 12-color LSR-II in early June.
Join our URMC Flow Cytometry Mailing List to keep updated with the latest developements in the Flow Core
Upcoming Events
Wednesday at 10am, MRB 3-9654
FRET Applications in Flow Cytometry: Theory and Practice
-1 December (Tuesday) at 1pm in 3-9654
Training Lecture - MC 3-6232
Nov 9, 30; Dec 7, 21
