Archives - 2012
30 November 2012
The training times through the rest of the year have been posted. Please be aware there is only one training lecture scheduled for December. Makre sure you can make it if you need it!
22 October 2012
I would like to welcome Cristy Bell and Terry Wightman to the staff of the Flow Core. Please feel free to ask them any questions related to the facility that you may have!
2 October 2012
I am pleased to congratulate Sharleen Slaunwhite who has passed the Certification exam in Cytometry.
For more information on the Certification Exam, go to http://cytometrycertification.org/index.html
1 October 2012
24 September 2012
Because of staffing changes, there will be some short-term changes to the calendar for the URMC FCC. We apologize in advance for any inconvenience and will work with you to minimize the impact of these short-term changes while we bring new staff up to speed.
Briefly through January 1st (or sooner if everyone is up to speed):
- All instruments will be down on Monday's till noon for the weekly long-cleaning and QC
- Sorters will shut down on Friday at 4pm
- Evening sort hours will only be available Tuesday-Thursday.
If you have any concerns, please contact either Matt Cochran or Tim Bushnell to discuss these short-term changes. We will be back to full complement as soon as possible.
20 September 2012
Dr Bunsen's Suggested Voltages have been updated to reflect the recent change in instrument baselines. I would also like to take this opportunity to welcome our newest muppet to duty, Rowlf. He is an 8-color LSRII residing in 3-4151.
18 September 2012
With the baselines being redone yesterday, all the Peak 2 information has also been updated. Our four LSRIIs (Fozzie, Anima, Kermit, and Gonzo) have their new information posted today. Bunsen will have his information posted tomorrow.
30 August 2012
I am pleased to announce that Matt Balys has passed the International Cytometry Certification Exam, and is now a Certified Cytometrist.
Congratulations to Matt on passing this exam!
To learn more about the ICCE, please go to http://cytometrycertification.org/index.html
22 August 2012
Today, a dear friend has left us. Beaker will no longer be part of the group at the core. But fear not! Some new friends will be coming online in the near future.
12 July 2012
The 9th annual Wester New York Flow User Group meeting is next Wednesday! Be sure to head over to our website and register to attend.
16 May 2012
With the holiday on May 28th, our training lecture was canceled. We have booked the same room, and same time, for training the day after, Tuesday May 29th!
14 May 2012
For those interested, there is an excel sheet highlighting the differences between our previous and new bead lots. It can be found from the Help Desk link regarding Peak 6 informaiton.
Also to note, out ninth annual meeting is coming up in July! If you would like to register, please use our registration link here. More information about the meeting can also be found at the link to the left.
7 May 2012
The next training lecture is May 14th. There will not be another training lecture in May as the 28th is a holiday. If there is a need for training before June, please let us know and we will try to accomodate!
4 May 2012
All new baselines have been run on our herd of Muppets. New Peak 2 voltages have also been run, and websites have been updated.
2 May 2012
To echo Tim's email sent earlier today about sorting:
As a reminder from our April 13th email to the list, we have implemented a
new sort form request that MUST be filled out and APPROVED before sorting.
On May 7th, if a sort has not been approved, the sort will not be performed
and you will be billed for the time reserved.
The form that Tim speaks of can be found here. Please be sure you are familiar with this form, and if you are not please contact either Tim or Matt to discuss the issues you are having.
23 April 2012
After all the work Fozzie had done last week, there was a need to redo his Peak 2 voltages. This information is now posted on his instrument page, and the new baseline is also in the library if needed.
13 April 2012
Over the weekend, our new database and sorting system will be rolled out. Please be sure to read the cytometry listserv announcement that Tim sent out earlier today. As always, feel free to stop by or let us know if you have any issues.
29 March 2012
With the recent work on Dr Bunsen, his voltages have been updated and the new baseline is available for some light reading in the library or from his instrument page.
26 March 2012
There is now an Accuri training document located on the library page of the website.
19 March 2012
The library page has been updated slightly to provide a question guided approach to locating information on the website. If there is something you routinely have to hunt for, please let a staff member know so the page can be updated!
2 March 2012
The next two training lecture dates have been posted. This coming Monday is the soonest. Also, there are two new instrument guides in the Library. One for FACSDiva, and one for the ImageStream. Give them a read if you are hungry for information!
6 February 2012
In an effort to streamline the data on intrument configuration pages, the linear ranges of PMTs has been removed and a link to each instrument's baseline report can now be found on the top of it's page.
3 February 2012
After a long delay, the seminar from January 9th is now available on the lectures page.
Also, two new forms have come online. The Change of Information form is for use then information tied to your account has changed. This would be account numbers, labs you work in, or if you are leaving the university. The second form is a Data Recovery Request. This form is to be used if you wish to recover data older than two months since it will no longer be on the local machines. Please fill out that form and the rest will be take care of. As always if you have any comments please feel free to contact a staff member. Thanks!
9 January 2012
Tuesday Tuesday Tuesday! Tomorrow in K-207 the Flow Core hosts the next seminar "Multicolor Flow Cytometry" with guest speaker Darci Gorgone from BD Biosciences.
Archives - 2011
This page will house old news and updates pertaining to the Flow Core here at the University of Rochester.
14 December 2011
There was a slight case of confusion with the voltages on Animal and Fozzie. This has been fixed and each instrument now has the correct numbers on their page.
7 December 2011
The next lecture presented by the Flow Core will be on Tuesday January 10th in K-207. Check the Lectures page for the abstract of this talk.
6 December 2011
With baselines being redone on all the instruments, new suggested voltages will also be present. These pages are located on the instruments themselves and will also be posted on the website shortly.
22 November 2011
Notice to all invesitgators that sort:
There is now a place in your reservation to fill in what nozzle you need for your sort. Please be sure to have this information filled in for future visits to best assist us with preparing for your arrival. The guideline for this new policy can be found on the sorting information page.
14 November 2011
The training dates through the end of the year have been posted. Please feel free to contact us if you have any questions about the dates listed. Thanks!
10 November 2011
As a note to all FlowJo users:
The price of dongles and dongle upgrades will be going up as of Jan 1st. Please be aware of this and see the following address for information on the price increase.
Old prices: http://www.flowjo.com/home/pricing.html
New prices: http://www.flowjo.com/home/prices2012.html
21 September 2011
The instructions for connecting to the new transfer share are now posted in the library. With the changing of the transfer service, the old pipeline should no longer be used and it will disappear shortly as well.
15 September 2011
Training lecture dates until the end of October have been posted. See you at the lecture!
7 July 2011
We have decided to branch out to other areas of the internet!
Now you can like us on Facebook!
6 July 2011
Fine sirs and madams of the flow community,
The instruments in the core have had their suggested voltages updated as a result of a new bead lot used for QC. Instrument pages now reflect the new values to use as of yesterday. Also, the newest baseline reports for each instrument can be found in the library if needed.
16 June 2011
The URMC FCC will be hosting a one-day training session on July 12th using FlowJo
software, run by a TreeStar applications scientist. There will be a morning session and an afternoon session.
You can register for either or both sessions at:https://www.surveymonkey.com/s/FlowJo
Registration will be capped at 20 people for each session, and priority will be given to labs who have registered with the FLS server.
1 June 2011
Due to popular demand, there will be a training lecture this coming Monday, June 6th. Same room and details as always, so head over to the Training page for more information.
26 May 2011
Since this coming Monday is a holiday there will be no training lecture until June 13.
9 May 2011
The time has come! Dr Bunsen Honeydew's esteemed assistant Beaker has arrived and is now operational. You can now find links to each FACSCantoII on the Instruments page. Configurations for each are different, so please be aware when yo are signing up to use one or the other.
4 May 2011
This message is in regards to the UR Cytometry mail that Tim sent out in the prior weeks:
In the near future, a new policy will be rolled out for the transfer pipeline. Any files older than two weeks will automatically be deleted. Please be aware of this and remove any files that you might need to prevent any issues when this happens.
28 April 2011
Tim recently taught a lecture for a graduate level course on Statistics and Cytometry. it has been posted to the Lecture page for those that are interested.
18 April 2011
Tim's next lecture in the Success in Cytometry series has been posted to the lecture page. Be sure to come here it live tomorrow Thursday, April 21st at 9:30 am in the Northeastern Conference room (1-9535/1-9525)
4 April 2011
Fozzie Bear has been fixed and there are new suggested voltages listed on his page. Be sure to check them out before your next run.
24 March 2011
Our esteemed Dr Bunsen Honeydew recently had some work done, and as a result new suggested voltages have been posted. Make sure to check the Analysis page to see what has been updated.
14 March 2011
New sort policy states that anything scheduled within 24 hours of a time slot must now be done through a staff member. Please call the core at x3-5085 to set up time if needed.
Gonzo was also on the fritz this morning. As a result, the database has been redone. This may lead to Application Specific Setting issues. Please contact Matt Cochran or Tim if you have any issues or concerns.
1 March 2011
The training lectures for the months of March and April have been listed. An upcoming lecture series by our core's director will be posted in the coming weeks so stay tuned for that.
5 January 2011
Happy new Year all! An exciting new training opportunity has just come to our attention and we would like to pass this information along to you. Please see the attached flyer for information.
Archives - 2010
27 December 2010
The voltages for all analytical instruments have been updated to reflect the recent change in CST bead lot. This means all voltages will be different from those use previously. Please be careful when using old experiments.
11 November 2010
As a note to users, all samples brought to the core must come in secondary containment. Styrofoam is not considered containment. This means even if samples are BSL-1, they must be contained properly.
9 November 2010
Two messages from Tim:
We are considering offering a week-long course at the URMC for
investigators to get trained in flow cytometry. The course would cover cell
preparation methods, data acquisition and analysis with flowjo, as well as
an introduction to others techniques (such as Luminex, the imagestream, cell
sorting and the like). Lecture topics would cover all aspects of flow
cytometry - from how the instrument works to designing experiments and many
topics in between. This course would cost about $400, to cover our costs,
and would be limited to 16 students.
The first course would be offered in January, if there is sufficient
interest. To gauge that interest, please contact me if you are interested,
and the level of interest (definitely take course, probably take course or
just interested) by November 19th.
The draft course outline can be found here.
I am pleased to announce that there will be a seminar on the new CyTOF cytometer on Friday, December 10th, at 12 noon in K-207 (2-6408). Dr. Vladimir Baranova will give a short talk on the technical aspects of this new technique. This will be followed by a longer biological applications talk by Dr. Olga Ornatsky.p>
This exciting new technology is one being considered by the flow core as a target for a Shared Instrument Grant in 2011, and the Rochester Human Immunology Center is working with the FCC and DVS (the makers of the CyTOF). There are only a few labs currently using this technology, and it brings the possibility of high-end (30+) marker detection to cytometry.p>
The abstract for the talk can be found here.
1 November 2010
The instruments page has been updated to better categorize the guidelines for our instruments.
The useful links page has also been updated and categorized.
26 October 2010
The sort request form has been removed from the Forms page. Instead of filling out the form, please know what Fluorochromes you are using and bring the list with you to each appointment. Thanks!
17 September 2010
The suggested voltages for Fozzie Bear have been updated to reflect recent instrument maintenance. Please be aware of these changes.
1 July 2010
Greetings all! With the new fiscal year starting today, the new hourly rates are in effect. Please head to the billing page to see the new rates.
15 March 2010
The lecture for Wednesday is posted.
23 February 2010
The lecture on compensation is up for tomorrow!
16 February 2010
The new biosafety recommendations page is now listed on the left. Please be sure to review all the information on the page. Be sure to contact a staff member if you have any questions.
15 February 2010
Wednesday's lecture is up and available for download on the Lectures page.
9 February 2010
The notes for Wednesday's lecture are now ready for download on the Lectures page.
5 February 2010
All of the instrument rooms have a phone now. The numbers are listed on the locations page.
2 February 2010
The lecture notes for tomorrow morning are posted on the Lectures page.
1 February 2010
A bit more information has been added to the sorting page and one additional guideline pertaining to the analytical instruments has been posted.
4 January 2010
With a new year comes new instrument locations and new baselines! All baselines, recommended voltages, and instrument locations have changed. Please see their respective pages for all the need to know.
Archives - 2009
25 November 2009
Please welcome our two new staff members, Ashley Adams and Jason Curran. They will be with us over in the Flow Core from now on.
20 November 2009
The training lecture for 23 November is being postponed a week to 30 November. Sorry for any inconvenience. Also, there will be a special lecture on 1 December on FRET presented by Dr Gabor Horvath in room 3-9654 at 1pm. The Lectures page will have details as they become available.
19 November 2009
With the holidays coming, there will be certain things going on around the Flow Core and RHIC. Please be aware of these things, especially those mentioned below:
- FACSCalibur retires 7 December.
- The LSRIIs in the RHIC move to the Flow Core 28-29 December. They will be down on these two days.
- Aria down 23 December through 4 January 2010.
18 November 2009
The lecture is up for today. Apologies for it being up far later than expected.
10 November 2009
Hello dear users! The guidelines for after hours use have been updated, so please make sure to get to the Instruments page and see what is new. Also, the Cyntellect seminar is tomorrow afternoon in the Louise Slaughter conference room. Check it out on the Lectures page.
26 October 2009
There is now a .pdf available for the Guava demo on November 3rd. Head to the Lecture page to download it.
7 October 2009
The lecture from this morning on multichromatic panel design can now be found on the Lecture page.
21 September 2009
Greetings all! We would like to inform all users that the 11 color LSRII in the RHIC is now a 12-color LSRII. A third PMT has been added to the violet laser. Please see the Analysis page for new voltages and filter configurations.
3 September 2009
Our newest instrument, the Amnis ImageStream GenX, is here and open for use! The relevant information is under Instruments > Imaging. Also, do not hesitate to ask Tim or Dave if you have any questions.
28 August 2009
The new Wednesday lecture series kicks off starting the 9th of September. See the right side for topics, location, and time. Also, the Accuri C6 cytometer is here! Please contact Tim if you would like more information about this instrument.
3 August 2009
The slides for the Introduction to Flow Lecture can be found on the Training page or by following this link.
29 June 2009
The FACSAria cell sorter has been moved to it's new home in the 3-4100 area! It is available for use again starting today.
27 May 2009
The sort form and guidelines for nozzle switching have been changed. Please be sure to familiarize yourself with the new information and the new sort form. As always, please let us know if you have any problems.
16 April 2009
The linear range of all PMTs and the suggested voltage is now listed for each instrument available in the Flow Core and RHIC. Please see the Analysis page for the values on each instrument.
14 April 2009
Oh boy, pictures! Tim's lecture for Thursday is now posted for your downloading enjoyment.
9 April 2009
The new fluorchrome naming policy was rolled out this past Monday. Please see the link for the new list in the library and of course do not hesitate to see any staff if you have a problem.
3 April 2009
The lecture for this Thursday's installment of Tim's lecture series is now available on the Lectures page!
1 April 2009
Tim's lecture for tomorrow is posted on the Lectures page. Have a look!
Also: There is now a Training link! This is the resource we will use from now on for all training information.
25 March 2009
The lecture for tomorrow has been posted. Grab it from the lectures page!
18 March 2009
The lecture for tomorrow's presentation by Tim has been posted on the lectures page. Get it while it's hot!
Addendum: I am sorry if anyone has tried getting the .pdf of the lecture for tomorrow, but the link was inadvertently broken most of the afternoon. It is now fixed. Thank you.
10 Febuary 2009
Dates for the lecture series have been posted. See the Lectures page for all the dates to save.
5 Febuary 2009
Lectures will now be posted to the Lectures link located in the navigation pane to the left of the page. Upcoming lectures will be announced here, as well as a backlog of all previous lectures, each available for download. Check back often as updates become available!
14 January 2009
The forms should once again be behind the firewall and working. We apologize for the downtime with the forms, but everything should be back to normal once again.
8 January 2009
As a follow-up to the UR Cytometry email sent by Tim, it should be noted that attendance of at least one of the listed lectures is mandatory for all users! If you do not attend, your account will be suspended. Please make sure you can come to one of these 30 minute lectures. Thank you.
5 January 2009
As a note to all users, the sort form, new user form, and cancellation form have been moved behind the firewall to prevent misuse. You will now need to have access to the Intranet by either being on a secure connection at the University or by using the VPN client to connect securely from a remote location. Thank you.
UPDATE: There is currently a problem with submission of the forms. If this happens, please let us know when you tried to submit the form and what browser you used during the process.
Archives - 2008
29 December 2008 - A message from Tim:
I am pleased to announce that the move from MBX 1-11314 to 3-4100 has gone smoothly. The 12-color LSR-II, FACSCanto, FACSCalibur and Imagestream all survived the trip and appear to be working well. We are in the process of validating and characterizing the instruments after their move and realignments.
Starting this week, we will have limited hours in the lab, based on staffing and such.
One important issue that is being addressed now is access to the 3-4100 area. At present and through at least 1/8/2009, the flow core will operate under reduced hours (9 am to 5:30 pm). The reason for this is that we do not have card-swipe access to our facility yet, and do not have sufficient keys to provide individual labs access. We are working with the keyshop to resolve this issue and hope to have card-swipe access on the main door and two analytical rooms (3-4151 and 3-4141) within 2 weeks.
Cell sorting will still be occurring in MRBX 1-11314, and we are working with Janet Ives to develop the new protocols that will be in place to comply with the IBC guidelines. Once those are finalized, I will notify the community as to them.
As always, if you have any questions or comments, please don’t hesitate to contact me directly.
Attention Flow Core users:
The core will be shutting down to move on December 22nd. ALL of the instruments in MRBX 1-11314 will be offline for that week. If there are any concerns, please talk to Tim sooner than later so that no problems that arise.
As a note to all Core and HIC user: There is now a reservation system for the HTS units that work in combination with any of the LSRIIs. These can be reserved in the same manner as an instrument found on the online calendar.
An update on the location of the Flow Core:
Renovations to the 3-4100 area are underway as of September 22nd. More information about a possible moving day will be posted as the time draws closer.
A note to users of the FACSVantage instruments:
These will be going offline as of August 1, 2008. Please make sure to familiarize yourself with the FACSAria and Diva software utilized by the Flow Core. It is also recommended that you validate any protocols on the FACSAria before experimentation is done. Please contact Tim if you have any further questions.
Additional information about the billing system in use has been added to the Billing link on the left panel. Please review the new information and speak with Tim if there are any questions or concerns.
The 12-color LSRII in the Flow Lab is now online! The laser configuration is the same as the 11-color LSRII in the HIC with the exception of the Violet laser. Head over to the Analysis page to find information about this difference. Reservations can be made here as well.
Our new calendar is on-line. Please bookmark http://coreserver.urmc-sh.rochester.edu/cgi-bin/flowcore/flowcore.pl. You will need a log-in to make reservations. You can apply for one here.
We will be bringing on-line a new 12-color LSR-II in early June.
Join our URMC Flow Cytometry Mailing List to keep updated with the latest developements in the Flow Core
Location/Time - Rm 3-4103 @ 4PM (in the flow core hallway)
Schedule: Dec 15th and 22nd, Jan 5th.
next lecture coming soon!
Comments about the Flow Core?