Animal Configuration

Suggested voltages current as of July 24, 2014.

Detailed information about this instrument can be found on the baseline report here.

Reservations

Reservations can be made online by following this link.

You will need an approved USER account. Apply for one here.

 

LSR-II Configuration: 

488 Laser  || 633 Laser  || 407 Laser || 532 Laser

BLUE 488 LASER (100mW):

Detector

LP

Band Pass

Fluor's

Suggested Voltage

BLUE A

685

710/50

PerCP-Cy5.5

550

BLUE B

505

515/20

FITC, CFSE, GFP, Alexa 488

550

BLUE C

  488/10 SSC  

FSC

 

RED 633 LASER (35mW):

Detector

LP

Band Pass

Fluor's

Suggested Voltage

RED A

740

780/60

APC-Cy7, APC-Alexa 750

550

RED B

685 710/50 APC-Cy5.5, Alexa 700, Alexa 680 500

RED C

660/20

APC, Alexa 647

550

VIOLET 407 LASER (65mW):

Detector

LP

Band Pass

Fluor's

Suggested Voltage

VIOLET A

740

780/60

Qdot 800, BV 786

600

VIOLET B

670

705/70

Qdot 705, BV 711

500

VIOLET C

630

660/40

Qdot 655, BV 650

600
VIOLET D 595 605/40 Qdot 605, BV 605 550
VIOLET E 570 585/42 Qdot 585 550
VIOLET F 557 560/40 Qdot 565 500
VIOLET G 535 550/40 Pacific Orange, BV 510, Qdot 545 500
VIOLET H   450/50 Pacific Blue, BV 421,Alexa 405 350

GREEN 532 LASER (50mW):

Detector

LP

Band Pass

Fluor's

Suggested Voltage

GREEN A

740

780/40

PE-Cy7, PE-Alexa 750

650

GREEN B

690

710/50

PE-Cy5.5

600

GREEN C

640 660/40 7AAD, PE-Cy5 550

GREEN D

600

610/20

PE-Alexa 610, PE-Texas Red

450

GREEN E

575/25

PE, PI, Alexa 532

400