Dr Bunsen Honeydew Configuration

Suggested voltages current as of July 24, 2014.

Detailed information about this instrument can be found in the baseline report here.

FACSCanto II Configuration: 

488 Laser  || 633 Laser  || 405 Laser

 

BLUE 488 LASER (20mW):

Detector

LP

Band Pass

Fluor's

Suggested Voltage

BLUE A

735

780/60

PE-Cy7

550

BLUE B

655

670LP

PerCP-Cy5.5, PE-Cy5.5, PE-Cy5, 7AAD, PerCP

550

BLUE C

610 - - -

BLUE D

556

585/42

PE, PI

450

BLUE E

502

530/30

FITC, GFP, YFP, AlexaFluor 488,

500

BLUE F

-

488/10

SSC

 
BLUE G - -    

BLUE H

---

-

RED 633 LASER (20mW):

Detector

LP

Band Pass

Fluor's

Suggested Voltage

RED A

735

780/60

APC-Cy7

550

RED B

685 -    

RED C

---

660/20

APC, Alexa 647, ToPro3

500

VIOLET 405 LASER (25mW):

Detector

LP

Band Pass

Fluor's

Suggested Voltage

VIOLET A

502

550/50

Pacific Orange, LiveDead Orange

450

VIOLET B

450/50

Pacific Blue, BV 421, DAPI, Vibrant Violet

350

VIOLET C

-

-