Cancer Center Instrumentation
The following instruments are for use by cancer center investigators only. If there is a circumstance where you believe you need to use one of these instruments and are not part of the cancer center, you must send an email to Tim Bushnell to explain your needs.
Reservations
Reservations can be made online by following this link. You will need an approved USER account. Apply for one here.
Cancer Center Sorter - Cancer Center LSRII
Cancer Center Sorter
Configuration:
488 Laser || 640 Laser || 405 Laser || 532 Laser || UV Laser
BLUE 488 LASER:
Detector |
LP |
Band Pass |
Fluor's |
BLUE A |
525 |
530/30 |
FITC, YFP |
BLUE B |
505 |
510/21 |
Alexa Fluor 488, GFP |
BLUE C |
|
488/10 |
SSC |
FSC |
|
|
|
|
RED 640 LASER
Detector |
LP |
Band Pass |
Fluor's |
RED A |
755 |
780/60 |
APC-Cy7, Alexa Fluor 750 |
RED B |
685 |
730/45 |
Alexa Fluor 700, APC-Alexa 700 |
RED C |
|
670/14 |
APC, Alexa 633 |
|
VIOLET 405 LASER
Detector |
LP |
Band Pass |
Fluor's |
VIOLET A |
670 |
705/70 |
Qdot 705 |
VIOLET B |
630 |
660/40 |
Qdot 655 |
| VIOLET C |
595 |
605/40 |
Qdot 605 |
| VIOLET D |
570 |
585/42 |
Qdot 585 |
| VIOLET E |
|
450/50 |
Pacific Blue |
|
GREEN 532 LASER
Detector |
LP |
Band Pass |
Fluor's |
GREEN A |
755 |
780/60 |
PE-Cy7 |
GREEN B |
685 |
710/50 |
PE-Cy5.5, PerCP-Cy5.5 |
GREEN C |
635 |
660/20 |
7AAD, PE-Cy5, PerCP |
GREEN D |
600 |
610/20 |
PE-Texas Red , PI, mCherry |
GREEN E |
550 |
575/25 |
PE, SYTOX Orange, RFP, tomato FP |
|
UV 355 LASER
Detector |
LP |
Band Pass |
Fluor's |
UV 1 |
505 |
530/30 |
Indo-1 (Blue), Pacific Orange |
UV 2 |
|
450/50 |
CFP, DAPI, Hoechst |
|
Cancer Center LSRII
Configuration:
488 Laser || 633 Laser || UV Laser
BLUE 488 LASER:
Detector |
LP |
Band Pass |
Fluor's |
BLUE A |
735 |
780/60 |
PE-Cy7 |
BLUE B |
685 |
695/40 |
PerCP-Cy5.5, PE-Cy5.5 |
BLUE C |
635 |
675/20 |
PE-Cy5, CyChrome, 7AAD |
BLUE D |
600 |
610/20 |
PE-TR, PI, DDAOG, DihEtbr |
BLUE E |
550 |
575/26 |
PE, DiL, JC-1 red, Mitotracker Red, RFP |
BLUE F |
525 |
530/30 |
FITC, Alexa 488, DCF, Thiazole Orange, YFP |
BLUE G |
505 |
510/20 |
GFP, DiO, DiOC6 |
BLUE H |
|
488/10 |
SSC |
FSC |
|
|
|
|
RED 633 LASER
Detector |
LP |
Band Pass |
Fluor's |
RED A |
735 |
780/60 |
APC-Cy7, APC-Alexa 750, DiD |
RED B |
685 |
730/45 |
APC-Cy5.5, APC-Alexa 700, Alexa 700 |
RED C |
|
660/20 |
APC, Alexa 633/647, DDAO, ToPor-3 |
|
UV 355 LASER
Detector |
LP |
Band Pass |
Fluor's |
UV A |
505 |
525/50 |
DAPI-UVA, mBBR, PacOrange |
UV B |
|
440/40 |
DAPI, Hoescht, Pac Blue |
|
