Cancer Center Instrumentation

The following instruments are for use by cancer center investigators only. If there is a circumstance where you believe you need to use one of these instruments and are not part of the cancer center, you must send an email to Tim Bushnell to explain your needs.

Reservations

Reservations can be made online by following this link. You will need an approved USER account. Apply for one here.

Cancer Center Sorter - Cancer Center LSRII

Cancer Center Sorter

Configuration: 

488 Laser  || 640 Laser  || 405 Laser || 532 Laser || UV Laser

BLUE 488 LASER:

Detector

LP

Band Pass

Fluor's

BLUE A

525

530/30

FITC, YFP

BLUE B

505

510/21

Alexa Fluor 488, GFP

BLUE C

  488/10 SSC

FSC

RED 640 LASER

Detector

LP

Band Pass

Fluor's

RED A

755

780/60

APC-Cy7, Alexa Fluor 750

RED B

685 730/45 Alexa Fluor 700, APC-Alexa 700

RED C

670/14

APC, Alexa 633

VIOLET 405 LASER

Detector

LP

Band Pass

Fluor's

VIOLET A

670

705/70

Qdot 705

VIOLET B

630

660/40

Qdot 655

VIOLET C 595 605/40 Qdot 605
VIOLET D 570 585/42 Qdot 585
VIOLET E   450/50 Pacific Blue

GREEN 532 LASER

Detector

LP

Band Pass

Fluor's

GREEN A

755

780/60

PE-Cy7

GREEN B

685

710/50

PE-Cy5.5, PerCP-Cy5.5

GREEN C

635 660/20 7AAD, PE-Cy5, PerCP

GREEN D

600

610/20

PE-Texas Red , PI, mCherry

GREEN E

550

575/25

PE, SYTOX Orange, RFP, tomato FP

UV 355 LASER

Detector

LP

Band Pass

Fluor's

UV 1

505

530/30

Indo-1 (Blue), Pacific Orange

UV 2

 

450/50

CFP, DAPI, Hoechst

Cancer Center LSRII

Configuration: 

488 Laser  || 633 Laser  || UV Laser

BLUE 488 LASER:

Detector

LP

Band Pass

Fluor's

BLUE A

735

780/60

PE-Cy7

BLUE B

685

695/40

PerCP-Cy5.5, PE-Cy5.5

BLUE C

635 675/20 PE-Cy5, CyChrome, 7AAD

BLUE D

600

610/20

PE-TR, PI, DDAOG, DihEtbr

BLUE E

550

575/26

PE, DiL, JC-1 red, Mitotracker Red, RFP

BLUE F

525

530/30

FITC, Alexa 488, DCF, Thiazole Orange, YFP

BLUE G

505

510/20

GFP, DiO, DiOC6

BLUE H

  488/10 SSC

FSC

RED 633 LASER

Detector

LP

Band Pass

Fluor's

RED A

735

780/60

APC-Cy7, APC-Alexa 750, DiD

RED B

685 730/45 APC-Cy5.5, APC-Alexa 700, Alexa 700

RED C

660/20

APC, Alexa 633/647, DDAO, ToPor-3

UV 355 LASER

Detector

LP

Band Pass

Fluor's

UV A

505

525/50

DAPI-UVA, mBBR, PacOrange

UV B

 

440/40

DAPI, Hoescht, Pac Blue