Gonzo Configuration

Suggested voltages current as of 13 March 2013.

Detailed information about this instrument can be found on the baseline report here.

LSR-II Configuration:

488 Laser || 633 Laser || 405 Laser

BLUE 488 LASER:

Detector	LP	Band Pass	Fluor's	Suggested Voltage
BLUE A	735	780/60	PE-Cy7, PE-Alexa750	600
BLUE B	685	710/50	PerCP-Cy5.5, PE-Cy5.5, PerCP, 7AAD, PE-Alexa700, PE-Alexa680	600
BLUE C	665	660/20	PE-Cy5, PE-Alexa647, 7AAD, Fura-Low_Ca++	550
BLUE D	600	610/20 630/30	PI, PE-Texas Red, PE-Alexa610, PE-Alexa594	600
BLUE E	550	576/26	PE	400
BLUE F	505	525/50	FITC, Alexa488, YFP, GFP, CFSE	500
BLUE G		488/10	SSC

RED 633 LASER

Detector	LP	Band Pass	Fluor's	Suggested Voltage
RED A	735	780/60	APC-Cy7, APC-Alexa750, APC-H7	650
RED B	685	710/50	APC-Cy5.5, AlexaFluor 700, AlexaFluor 680	550
RED C		660/20	APC, Alexa 647	500

VIOLET 405 LASER

Detector	LP	Band Pass	Fluor's	Suggested Voltage
VIOLET A	595	610/20	Qdot 605	500
VIOLET B	505	525/50	AlexaFluor 430, CFP, Fura-Hich_Ca++, Pacific Orange	450
VIOLET C		450/50	AlexaFluor 405, DAPI, Marina Blue, Pacific Blue, Sytox Blue	450