Kermit Configuration

Suggested voltages current as of 11 March 2013.

Detailed information about this instrument can be found on the baseline report here.

 

LSR-II Configuration: 

488 Laser  || 633 Laser  || 405 Laser

BLUE 488 LASER (20mW):

Detector

LP

Band Pass

Fluor's

Suggested Voltage

BLUE A

735

780/60

PE-Cy7, PE-Alexa750

550

BLUE B

685

710/50

PerCP-Cy5.5, PE-Cy5.5, PerCP, PE-Alexa700, PE-Alexa680

550

BLUE C

665 660/20 PE-Cy5, PE-Alexa647, 7AAD, Fura-Low_Ca++ 600

BLUE D

600

610/20

630/30

PI, PE-Texas Red, PE-Alexa610, PE-Alexa594

550

BLUE E

550

576/26

PE

550

BLUE F

505

525/50

FITC, Alexa488, YFP, GFP, CFSE

500
BLUE G   488/10 SSC  

RED 633 LASER (20mW):

Detector

LP

Band Pass

Fluor's

Suggested Voltage

RED A

735

780/60

APC-Cy7, APC-Alexa750, APC-H7

550

RED B

685 710/50 APC-Cy5.5, AlexaFluor 700, AlexaFluor 680 450

RED C

660/20

APC, Alexa 647

400

VIOLET 405 LASER (40mW):

Detector

LP

Band Pass

Fluor's

Suggested Voltage

VIOLET A

595

610/20

Qdot 605

550

VIOLET B

505

525/50

AlexaFluor 430, CFP, Fura-Hich_Ca++, Pacific Orange

500

VIOLET C

450/50

AlexaFluor 405, DAPI, Marina Blue, Pacific Blue, Sytox Blue

400