Waldorf Configuration

Suggested voltages current as of 25 March 2013.

Reservations

Reservations can be made online by following this link.

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Configuration: 

488 Laser  || 633 Laser  || 407 Laser || 532 Laser

BLUE 488 LASER (100mW):

Detector

LP

Band Pass

Fluor's

Suggested Voltage

BLUE A

670

685/35

PerCP-Cy5.5

550

BLUE B

505

525/50

FITC, CFSE, GFP, Alexa 488

500

BLUE C

  488/10 SSC  

FSC

 

RED 633 LASER (40mW):

Detector

LP

Band Pass

Fluor's

Suggested Voltage

RED A

740

780/60

APC-Cy7, APC-Alexa 750

500

RED B

690 730/45 APC-Cy5.5, Alexa 700, Alexa 680 450

RED C

660/20

APC, Alexa 647

500

VIOLET 407 LASER (100mW):

Detector

LP

Band Pass

Fluor's

Suggested Voltage

VIOLET A

740

780/60

Qdot 800

650

VIOLET B

670

705/70

Qdot 705

600

VIOLET C

630

670/30

Qdot 655

550
VIOLET D 595 605/40 Qdot 605 550
VIOLET E 570 585/42 Qdot 585 500
VIOLET F 557 560/40 Qdot 565 450
VIOLET G 505 525/50 Pacific Orange, Alexa 430, Qdot 545 400
VIOLET H   450/50 Pacific Blue, Alexa 405, Cascade Blue 400

GREEN 532 LASER (150mW):

Detector

LP

Band Pass

Fluor's

Suggested Voltage

GREEN A

750

780/60

PE-Cy7, PE-Alexa 750

600

GREEN B

690

710/50

PE-Cy5.5

450

GREEN C

635 660/20 7AAD, PE-Cy5 400

GREEN D

600

610/20

PE-Alexa 610, PE-Texas Red

400

GREEN E

575/25

PE, Alexa 532

400