Library

The following reference materials are available for researchers to borrow:

Methods in Cell Biology, Volume 75: Cytometry 4th Edition. New Developments. (2004) Darzynkiewick, Roederer and Tanke (eds)
Current Protocols in Flow Cytometry
Course Notebook - 27th Annual Course in Flow Cytometry
PROTOCOLS

These flow protocols have been validated by members of the Flow Core.

Connecting to the Flow Core server

This is only needed if you are at a location outside of the University.

If so, you need the VPN client .

For Mac:

Go > Connect to Server... > smb://flowcore.urmc-sh.rochester.edu

Choose the TransferPipeline as volume to mount

For PC:

Start Menu > Run... > \\flowcore.urmc-sh.rochester.edu

Choose the TransferPipeline folder

Fluorchrome List

Fluorchrome List - Need to log in to access file.

This list represents the fluorchromes we use at the Flow Core. It takes effect 6 April 2009 with the change to the PMT naming policy rolled out the same day.

Suggested Voltage List

Full List for all instruments -September 2009

Current Instrument Baseline

Recent Publications

Enos, M., Bancos, S., Bushnell, T., and Crispe, I.N. (2008) E2F4 modulates differentiation and gene expression in hematopoietic progenitor cells during commitment to the lymphoid lineage J Immunol 180:3699-707
McGrath, K.E., Kingsley, P.D., Koniski, A..D., Porter, R.L., Bushnell, T.P., and Palis, J.(2007) Enucleation of primitive erythroid cells generates a transient population of "pyrenocytes" in the mammalian fetus. Blood 111:2409-17
Guzman, M.L., Li, X., Corbett, C.A., Rossi, R.M., Bushnell, T., Liesveld J.L., Herbet, J., Young, F., and Jordan C.T. (2007) Rapid and selective death of leukemia stem and progenitor cells induced by the compound 4-benzyl, 2-methyl, 1,2,4-thiadiazolidine, 3,5 dione (TDZD-8). Blood 110:4436-44
Ryan, E.P., Bushnell, T.P., Friedman, A.E., Rahman, I., and Phipps, R.P. (2007) Cyclooxygenase-2 selective inhibition reduces B lymphoma survival by inducing oxidative stress and decreasing intracellular glutathione Cancer Immunol Immunother 57:347-58
Neering, S.J., Bushnell, T., Sozer, S., Ashton, J., Rossi, R.M., Wang, P.-Y., Bell, D.R., Heinrich, D., Bottaro, A, and Jordan C.T. (2007) Leukemia stem cells in a genetically defined murine model of blast crisis CML. Blood 110:2578-85.
Graf, B., Bushnell, T., and Miller , J. (2007) LFA-1-mediated T cell costimulation through increased localization of TCR/class II complexes to the cSMAC and exclusion of CD45 from the immunological synapse. J Immunol., 179:1616-24
Neff-LaFord, H., Teske, S., Bushnell, T.P., and Lawrence, B.P. (2007) Aryl hydrocarbon receptor activation during influenza virus infection unveils a novel pathway of IFN-a production by phagocytic cells J Immunol 179:247-55
Kingsley, PD, Malik J, Emerson RL, Bushnell TP, McGrath KE, Bloedorn LA, Bulger M, and Palis J (2006) “Maturational” globin switching in primary primitive erythroid cells. Blood 107:1665-1672

Upcoming Events

Wednesday at 10am, MRB 3-9654

10/14 Multichromatic Panel Design: Practical

10/28 An Introduction to Fluorescence Microscopy

11/3 - Tuesday at 11AM Introducing the Guava 8HT personal flow cytometer

Training Lecture - MC 3-6232

Sept 28, Oct 12, Oct 26