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Sort Request Form

Please make sure to check and complete all information before submitting this form. If you have any questions, please contact Tim Bushnell to set up an appointment to discuss your needs and application. Incomplete or missing information will result in the form being returned to you and your sort time not being approved until the form is properly filled out.

Aria Sort Information

General Information

Researcher Name: Principle Investigator: Account Number: Proposed date of experiment

Sample Information

Primary cells:

Human Mouse Rat Other

Cell Lines:

Human Mouse Rat Other

If the cell type is 'Other', please indicate the species origin, cell line name or other identifying information here:

Other Cell Line:

Have the cells been tested for Pathogens?

No Yes
If "Yes" indicate the pathogen(s) tested for and the results of the test:

Have the cells been tested for Mycoplasma?

No Yes
If "Yes" indicate date: and results Positive Negative

Sample Biosafety Level

Please select a Biosafety Level BSL1 BSL2 BSL3

Is the sample transformed or infected with a virus, nucleic acid or virus vector or other pathogen? Yes No If YES, then please provide details of the pathogen including MSDS information:

Does the PI have IBC approval to possess and use the cells and/or virus: No Yes

All requests for sorts involving BSL-2 level organisms and/or infected samples requiring IBC approval will be verified with the IBC before sort time can be approved. This can take up to 48 hours. If the PI does not have approval from the IBC for possession of the cells and/or pathogen, the sort time will be canceled.

Fluorochromes

488 Laser

Blue A: Pe-Cy7

Blue B: Pe-Cy5.5 PerCp-Cy5.5

Blue C: 7-AAD Pe-Cy5 PerCp

Blue D: PE-Texas Red Propidium Iodide PE-Alexa610

Blue E: PE

Blue F: FITC Thiazole Orange Alexa 488 Alexa 430 YFP

Blue G: GFP

633 Laser

Red A: APC-Cy7 APC-Ax750

Red B: APC-Cy5.5 APC-Ax700

Red C: APC Alexa 660 To-Pro3

407 Laser

Violet A: Ax430 Qdot520 Pacific Orange

Violet B: Hoescht CFP

Violet C: DAPI Alexa 405 Cascade Blue Pacific Blue

Non-standard Fluorochromes

If your fluorochromes is not on this list, please indicate below what fluorochrome(s) you are using.

Sample Output

Collection Method

Temperature Setting Room Temp 4°C 20°C 37°C 42°C

Sort Speed Low (20 PSI) Medium (35 PSI) High (70 PSI)

Sort Analysis only 2 way: 5 ml tubes 2 way: 15 ml tubes 4 way: 1.5 ml tubes 4 way: 5 ml tubes Plate: 6 well Plate: 12 well Plate: 24 well Plate: 48 well Plate: 96 well Plate: 384 well Terasaki 6x10 Terasaki 6x12 Slide

Additional Questions

Is the sample filtered?

No Yes

Is the sample sterile?

No Yes

Does sort need to be sterile?

No Yes

Is the sample treated with DNAse?

No Yes

Approximate size range of your cells

Below 10 microns 10-20 microns Larger than 20 microns

Is the sample an adherent cell line?

No Yes

If yes, were the cells removed with trypsin?

No Yes

If cells treated with trypsin, how was trypsin inhibited?

Serum Trypsin Inhibitor other