Flow Cytometry Training Center

Welcome to Flow Cytometry! Below you will find the rough outline for how we train new users to th Flow Core here at the University of Rochester.

New to the Flow Core?

The following documents will either be needed during training or are a useful resource before you come for training.

  1. Introduction to Flow
  2. BD FACSCanto II User Information
  3. BD LSRII User Information
  4. BD LSRII HTS User Information

Registration

Everyone must register for a user account and flow training if they would like to use the Flow Core instruments. This is done by:
  1. Fill out the New User Form. The new user will be contacted regarding the beginning of the training process by the Flow Core staff.
  2. Read and complete the Intro to Flow tutorial.
  3. Read and print a copy of the FACSCantoII Quickstart Guide.

After Registration

Introduction to Flow Lecture.

Before you can sign up for initial training, you will be required to attend the Introduction to Flow lecture. This is a 1 hour basic lecture to introduce you to the concepts of flow cytometry. Training will occur in 3-6232, the McCann A room at 4 pm every other week. Please be sure to have reviewed the material in the ‘Introduction to Flow’ pdf.

Follow this link to get the slides for the Introduction lecture.

First Training Visit

The first visit is done on the FACSCantoII located in the Flow Core using beads and will serve as an introduction to flow cytometry and the FACSDiva software. The trainer will perform the setup and run samples with the new user watching.

Training sessions only occur Wednesday-Friday mornings, 9-11am.

  1. Bring a copy of the FACSCantoII Quickstart Guide.
  2. Ask any questions brought up while reading the Introduction to Flow tutorial.
  3. A basic experiment will be run from start to finish including compensation.
  4. There is no charge for this training visit.

Second Training Visit

The new user will run from start to finish with guidance throughout from the trainer on the instrument that will be used for future work.
  1. Running practice samples is suggested prior to running critical samples.
  2. Schedule a time with a member of the training staff so assistance can be provided.
  3. Bring the quickstart guide for the instrument to be used. The guide for the FACSCantoII and LSRII found here.
  4. There is no charge for this training visit.

Visits 3-6

For the first four solo runs on the Flow Core instruments it is required that a certified member of the Flow Core staff be available.
  1. Time can be scheduled at any point during normal business hours (Mon-Fri 830am-530pm)
  2. New users will need to be signed into the FACSDiva software by a member of the staff.
  3. The new user will run the samples with assistance as needed from the staff.
  4. Visits 3-6 are billed at the normal hourly rate.
  5. If additional training is needed after visit 6 an appropriate course of action will be determined.

After Hours Access

The Flow Core is open during normal business hours (9am to 5pm) from Monday through Friday. If you need access after hours or on the weekend, please fill out the after hours access form. It will take approximately 2 weeks from submission of the form until acccess is granted, so please plan accordingly.

Helpful Training Links

http://www.bdbiosciences.com/immunocytometry_systems/support/training/online/ITF/index.shtml

http://www.bdbiosciences.com/immunocytometry_systems/support/training/online/diva6_course/

http://www.bdbiosciences.com/immunocytometry_systems/support/training/online/canto6_course/

Upcoming Events

Wednesday at 10am, MRB 3-9654

10/14 Multichromatic Panel Design: Practical

10/28 An Introduction to Fluorescence Microscopy

11/3 - Tuesday at 11AM Introducing the Guava 8HT personal flow cytometer

Training Lecture - MC 3-6232

Sept 28, Oct 12, Oct 26