Research Projects
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Understanding of Hole and Excess-Electron Trapping and Detrapping by DNA
Hypothesis that are currently being tested are, radical combination reactions, persisting hours after energy deposition at 4K, are propagated by tunneling, thermally activated detrapping, of radicals stably trapped at 4K, is
gated
by reversible proton transfer, etc. More info... -
Reactions that Lead to the Formation of Radicals that are Trapped in DNA
Radical trapping and reactions are being measured in a series of DNA crystals, testing our working model. Imbedded in the model are a number of specific hypotheses. More info...
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Correlate Stable End Products with Their Free Radical Precursors
The hypothesis that all (>99%) of the stable end products produced in DNA are derived from free radical precursors is being tested and the reaction pathways elucidated. For crystalline DNA irradiated at 4K, the prediction that the ratio of strand breaks to base damage is unusually large (about 1:1) will be tested. More info...
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Detect Multiply Damaged Sites and Determine Their Yields
These experiments are designed to optimize the chances of measuring the yields of specific types of multiply damaged sites. They test, among other things, the hypothesis that some stable sites of reductive damage are spatially correlated with a nearby site of sugar oxidation. More info...
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Size Based Filtration of Oligodeoxynucleotides
The unique characteristics of SimPore filters make it possible to address key problems addressing the mechanism by which ionizing radiation damages DNA via the direct effect. One is to use the filters to purify oligodeoxynucleotides. More info...
