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Principal Investigator

Philip J. Fay, Ph.D. University of Rochester work Box 712 601 Elmwood Ave Rochester NY 14642 office: MC 3-8553 p 585-275-6756 f 585-473-4314 Philip_Fay@urmc.rochester.edu

Recent Publications

The following list was auto-generated 11 hours ago.

• 1. Wakabayashi H
  2. Fay PJ
  (2013 Jun 01). Molecular orientation of Factor VIIIa on the phospholipid membrane surface determined by fluorescence resonance energy transfer. Biochem J. 452, 293-301.
• 1. Wakabayashi H
  2. Fay PJ
  (2013 May 09). Modification of inter-domain interfaces within the A3C1C2 subunit of factor VIII affects its stability and activity. Biochemistry. In press.
• 1. Griffiths AE
  2. Rydkin I
  3. Fay PJ
  (2013 Apr 11). Factor VIIIa A2 Subunit Shows a High Affinity Interaction with Factor IXa: Contribution of A2 Subunit Residues 707-714 to the Interaction with Factor IXa. J Biol Chem. In press.
• 1. Takeyama M
  2. Wintermute JM
  3. Manithody C
  4. Rezaie AR
  5. Fay PJ
  (2013 Apr 02). Variable contributions of basic residues forming an APC exosite in the binding and inactivation of factor VIIIa. Biochemistry. 52, 2228-35.
• 1. Yada K
  2. Nogami K
  3. Wakabayashi H
  4. Fay PJ
  5. Shima M
  (2013 Mar 07). The mild phenotype in severe hemophilia A with Arg1781His mutation is associated with enhanced binding affinity of factor VIII for factor X. Thromb Haemost. 109, .
• 1. Takeyama M
  2. Wakabayashi H
  3. Fay PJ
  (2013 Feb 01). Contribution of factor VIII light-chain residues 2007-2016 to an activated protein C-interactive site. Thromb Haemost. 109, 187-98.
• 1. DeAngelis JP
  2. Wakabayashi H
  3. Fay PJ
  (2012 May 04). Sequences flanking Arg336 in factor VIIIa modulate factor Xa-catalyzed cleavage rates at this site and cofactor function. J Biol Chem. 287, 15409-17.
• 1. Newell-Caito JL
  2. Griffiths AE
  3. Fay PJ
  (2012 Apr 24). P3-P3' residues flanking scissile bonds in factor VIII modulate rates of substrate cleavage and procofactor activation by thrombin. Biochemistry. 51, 3451-9.
• 1. Wakabayashi H
  2. Griffiths AE
  3. Fay PJ
  (2012 Mar 01). Enhancing factor VIII and VIIIa stability by combining mutations at the A2 domain interface and A1-C2 domain interface. J Thromb Haemost. 10, 492-5.
• 1. Takeyama M
  2. Wakabayashi H
  3. Fay PJ
  (2012 Jan 24). Factor VIII light chain contains a binding site for factor X that contributes to the catalytic efficiency of factor Xase. Biochemistry. 51, 820-8.
• 1. DeAngelis JP
  2. Varfaj F
  3. Wakabayashi H
  4. Fay PJ
  (2011 Nov 01). The role of P4-P3' residues flanking Arg336 in facilitating activated protein C-catalyzed cleavage and inactivation of factor VIIIa. Thromb Res. 128, 470-6.
• 1. Griffiths AE
  2. Wang W
  3. Hagen FK
  4. Fay PJ
  (2011 Aug 01). Use of affinity-directed liquid chromatography-mass spectrometry to map the epitopes of a factor VIII inhibitor antibody fraction. J Thromb Haemost. 9, 1534-40.
• 1. Wakabayashi H
  2. Griffiths AE
  3. Fay PJ
  (2011 Jul 22). Increasing hydrophobicity or disulfide bridging at the factor VIII A1 and C2 domain interface enhances procofactor stability. J Biol Chem. 286, 25748-55.
• 1. Novakovic VA
  2. Cullinan DB
  3. Wakabayashi H
  4. Fay PJ
  5. Baleja JD
  6. Gilbert GE
  (2011 Apr 01). Membrane-binding properties of the Factor VIII C2 domain. Biochem J. 435, 187-96.
• 1. Wakabayashi H
  2. Griffiths AE
  3. Fay PJ
  (2010 Aug 13). Factor VIII lacking the C2 domain retains cofactor activity in vitro. J Biol Chem. 285, 25176-84.
• 1. Jagannathan I
  2. Ichikawa HT
  3. Kruger T
  4. Fay PJ
  (2009 Nov 20). Identification of residues in the 558-loop of factor VIIIa A2 subunit that interact with factor IXa. J Biol Chem. 284, 32248-55.
• 1. Newell JL
  2. Fay PJ
  (2009 Apr 24). Cleavage at Arg-1689 influences heavy chain cleavages during thrombin-catalyzed activation of factor VIII. J Biol Chem. 284, 11080-9.
• 1. Wakabayashi H
  2. Griffiths AE
  3. Fay PJ
  (2009 Mar 01). Combining mutations of charged residues at the A2 domain interface enhances factor VIII stability over single point mutations. J Thromb Haemost. 7, 438-44.
• 1. Wakabayashi H
  2. Varfaj F
  3. Deangelis J
  4. Fay PJ
  (2008 Oct 01). Generation of enhanced stability factor VIII variants by replacement of charged residues at the A2 domain interface. Blood. 112, 2761-9.
• 1. Newell JL
  2. Fay PJ
  (2008 Aug 19). Acidic residues C-terminal to the A2 domain facilitate thrombin-catalyzed activation of factor VIII. Biochemistry. 47, 8786-95.
• 1. Wakabayashi H
  2. Fay PJ
  (2008 Apr 25). Identification of residues contributing to A2 domain-dependent structural stability in factor VIII and factor VIIIa. J Biol Chem. 283, 11645-51.
• 1. Newell JL
  2. Fay PJ
  (2007 Aug 31). Proteolysis at Arg740 facilitates subsequent bond cleavages during thrombin-catalyzed activation of factor VIII. J Biol Chem. 282, 25367-75.
• 1. Varfaj F
  2. Wakabayashi H
  3. Fay PJ
  (2007 Jul 13). Residues surrounding Arg336 and Arg562 contribute to the disparate rates of proteolysis of factor VIIIa catalyzed by activated protein C. J Biol Chem. 282, 20264-72.
• 1. Wakabayashi H
  2. Zhou Q
  3. Varfaj F
  4. Fay PJ
  (2007 May 01). A3 domain residue Glu1829 contributes to A2 subunit retention in factor VIIIa. J Thromb Haemost. 5, 996-1001.
• 1. Ansong C
  2. Miles SM
  3. Fay PJ
  (2006 Nov 07). Factor VIII A1 domain residues 97-105 represent a light chain-interactive site. Biochemistry. 45, 13140-9.
• 1. Wakabayashi H
  2. Zhou Q
  3. Nogami K
  4. Ansong C
  5. Varfaj F
  6. Miles S
  7. Fay PJ
  (2006 Jun 01). pH-dependent association of factor VIII chains: enhancement of affinity at physiological pH by Cu2+. Biochim Biophys Acta. 1764, 1094-101.
• 1. Varfaj F
  2. Neuberg J
  3. Jenkins PV
  4. Wakabayashi H
  5. Fay PJ
  (2006 Jun 01). Role of P1 residues Arg336 and Arg562 in the activated-Protein-C-catalysed inactivation of Factor VIIIa. Biochem J. 396, 355-62.
• 1. Ansong C
  2. Miles SM
  3. Fay PJ
  (2006 Apr 01). Epitope mapping factor VIII A2 domain by affinity-directed mass spectrometry: residues 497-510 and 584-593 comprise a discontinuous epitope for the monoclonal antibody R8B12. J Thromb Haemost. 4, 842-7.
• 1. Fay PJ
  (2006 Feb 01). Factor VIII structure and function. Int J Hematol. 83, 103-8.
• 1. Wakabayashi H
  2. Su YC
  3. Ahmad SS
  4. Walsh PN
  5. Fay PJ
  (2005 Aug 02). A Glu113Ala mutation within a factor VIII Ca2+-binding site enhances cofactor interactions in factor Xase. Biochemistry. 44, 10298-304.
• 1. Ansong C
  2. Fay PJ
  (2005 Jun 21). Factor VIII A3 domain residues 1954-1961 represent an A1 domain-interactive site. Biochemistry. 44, 8850-7.
• 1. Nogami K
  2. Zhou Q
  3. Wakabayashi H
  4. Fay PJ
  (2005 Jun 01). Thrombin-catalyzed activation of factor VIII with His substituted for Arg372 at the P1 site. Blood. 105, 4362-8.
• 1. Nogami K
  2. Zhou Q
  3. Myles T
  4. Leung LL
  5. Wakabayashi H
  6. Fay PJ
  (2005 May 06). Exosite-interactive regions in the A1 and A2 domains of factor VIII facilitate thrombin-catalyzed cleavage of heavy chain. J Biol Chem. 280, 18476-87.
• 1. Fay PJ
  2. Jenkins PV
  (2005 Jan 01). Mutating factor VIII: lessons from structure to function. Blood Rev. 19, 15-27.
• 1. Nogami K
  2. Wakabayashi H
  3. Ansong C
  4. Fay PJ
  (2004 Sep 01). Localization of a pH-dependent, A2 subunit-interactive surface within the factor VIIIa A1 subunit. Biochim Biophys Acta. 1701, 25-35.
• 1. Nogami K
  2. Freas J
  3. Manithody C
  4. Wakabayashi H
  5. Rezaie AR
  6. Fay PJ
  (2004 Aug 06). Mechanisms of interactions of factor X and factor Xa with the acidic region in the factor VIII A1 domain. J Biol Chem. 279, 33104-13.
• 1. Jenkins PV
  2. Dill JL
  3. Zhou Q
  4. Fay PJ
  (2004 May 04). Contribution of factor VIIIa A2 and A3-C1-C2 subunits to the affinity for factor IXa in factor Xase. Biochemistry. 43, 5094-101.
• 1. Nogami K
  2. Lapan KA
  3. Zhou Q
  4. Wakabayashi H
  5. Fay PJ
  (2004 Apr 16). Identification of a factor Xa-interactive site within residues 337-372 of the factor VIII heavy chain. J Biol Chem. 279, 15763-71.
• 1. Wakabayashi H
  2. Freas J
  3. Zhou Q
  4. Fay PJ
  (2004 Mar 26). Residues 110-126 in the A1 domain of factor VIII contain a Ca2+ binding site required for cofactor activity. J Biol Chem. 279, 12677-84.
• 1. Jenkins PV
  2. Dill JL
  3. Zhou Q
  4. Fay PJ
  (2004 Mar 01). Clustered basic residues within segment 484-510 of the factor VIIIa A2 subunit contribute to the catalytic efficiency for factor Xa generation. J Thromb Haemost. 2, 452-8.
• 1. Fay PJ
  (2004 Mar 01). Activation of factor VIII and mechanisms of cofactor action. Blood Rev. 18, 1-15.
• 1. Manithody C
  2. Fay PJ
  3. Rezaie AR
  (2003 Jun 15). Exosite-dependent regulation of factor VIIIa by activated protein C. Blood. 101, 4802-7.
• 1. Nogami K
  2. Wakabayashi H
  3. Fay PJ
  (2003 May 09). Mechanisms of factor Xa-catalyzed cleavage of the factor VIIIa A1 subunit resulting in cofactor inactivation. J Biol Chem. 278, 16502-9.
• 1. Balakrishnan M
  2. Roques BP
  3. Fay PJ
  4. Bambara RA
  (2003 Apr 01). Template dimerization promotes an acceptor invasion-induced transfer mechanism during human immunodeficiency virus type 1 minus-strand synthesis. J Virol. 77, 4710-21.
• 1. Chen Y
  2. Balakrishnan M
  3. Roques BP
  4. Fay PJ
  5. Bambara RA
  (2003 Mar 07). Mechanism of minus strand strong stop transfer in HIV-1 reverse transcription. J Biol Chem. 278, 8006-17.
• 1. Nogami K
  2. Wakabayashi H
  3. Schmidt K
  4. Fay PJ
  (2003 Jan 17). Altered interactions between the A1 and A2 subunits of factor VIIIa following cleavage of A1 subunit by factor Xa. J Biol Chem. 278, 1634-41.
• 1. Wakabayashi H
  2. Zhen Z
  3. Schmidt KM
  4. Fay PJ
  (2003 Jan 14). Mn2+ binding to factor VIII subunits and its effect on cofactor activity. Biochemistry. 42, 145-53.
• 1. Roda RH
  2. Balakrishnan M
  3. Kim JK
  4. Roques BP
  5. Fay PJ
  6. Bambara RA
  (2002 Dec 06). Strand transfer occurs in retroviruses by a pause-initiated two-step mechanism. J Biol Chem. 277, 46900-11.
• 1. Jenkins PV
  2. Freas J
  3. Schmidt KM
  4. Zhou Q
  5. Fay PJ
  (2002 Jul 15). Mutations associated with hemophilia A in the 558-565 loop of the factor VIIIa A2 subunit alter the catalytic activity of the factor Xase complex. Blood. 100, 501-8.
• 1. Wakabayashi H
  2. Schmidt KM
  3. Fay PJ
  (2002 Jul 02). Ca(2+) binding to both the heavy and light chains of factor VIII is required for cofactor activity. Biochemistry. 41, 8485-92.