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Principal Investigator

Eric Phizicky, Ph.D. University of Rochester work Box 712 Rochester NY p 585-275-7268 f 585-271-2683 eric_phizicky@urmc.rochester.edu

Recent Publications

The following list was auto-generated 2 days ago.

• 1. Grant TD
  2. Luft JR
  3. Wolfley JR
  4. Snell ME
  5. Tsuruta H
  6. Corretore S
  7. Quartley E
  8. Phizicky EM
  9. Grayhack EJ
  10. Snell EH
  (2013 Apr 10). The Structure of Yeast Glutaminyl-tRNA Synthetase and Modeling of Its Interaction with tRNA. J Mol Biol. In press.
• 1. Preston MA
  2. D'Silva S
  3. Kon Y
  4. Phizicky EM
  (2013 Feb 01). tRNAHis 5-methylcytidine levels increase in response to several growth arrest conditions in Saccharomyces cerevisiae. RNA. 19, 243-56.
• 1. Guy MP
  2. Podyma BM
  3. Preston MA
  4. Shaheen HH
  5. Krivos KL
  6. Limbach PA
  7. Hopper AK
  8. Phizicky EM
  (2012 Oct 01). Yeast Trm7 interacts with distinct proteins for critical modifications of the tRNAPhe anticodon loop. RNA. 18, 1921-33.
• 1. Dewe JM
  2. Whipple JM
  3. Chernyakov I
  4. Jaramillo LN
  5. Phizicky EM
  (2012 Oct 01). The yeast rapid tRNA decay pathway competes with elongation factor 1A for substrate tRNAs and acts on tRNAs lacking one or more of several modifications. RNA. 18, 1886-96.
• 1. Grant TD
  2. Snell EH
  3. Luft JR
  4. Quartley E
  5. Corretore S
  6. Wolfley JR
  7. Snell ME
  8. Hadd A
  9. Perona JJ
  10. Phizicky EM
  11. Grayhack EJ
  (2012 Apr 01). Structural conservation of an ancient tRNA sensor in eukaryotic glutaminyl-tRNA synthetase. Nucleic Acids Res. 40, 3723-31.
• 1. Wilusz JE
  2. Whipple JM
  3. Phizicky EM
  4. Sharp PA
  (2011 Nov 11). tRNAs marked with CCACCA are targeted for degradation. Science. 334, 817-21.
• 1. Whipple JM
  2. Lane EA
  3. Chernyakov I
  4. D'Silva S
  5. Phizicky EM
  (2011 Jun 01). The yeast rapid tRNA decay pathway primarily monitors the structural integrity of the acceptor and T-stems of mature tRNA. Genes Dev. 25, 1173-84.
• 1. D'Silva S
  2. Haider SJ
  3. Phizicky EM
  (2011 Jun 01). A domain of the actin binding protein Abp140 is the yeast methyltransferase responsible for 3-methylcytidine modification in the tRNA anti-codon loop. RNA. 17, 1100-10.
• 1. Phizicky EM
  2. Hopper AK
  (2010 Sep 01). tRNA biology charges to the front. Genes Dev. 24, 1832-60.
• 1. Preston MA
  2. Phizicky EM
  (2010 May 01). The requirement for the highly conserved G-1 residue of Saccharomyces cerevisiae tRNAHis can be circumvented by overexpression of tRNAHis and its synthetase. RNA. 16, 1068-77.
• 1. Murthi A
  2. Shaheen HH
  3. Huang HY
  4. Preston MA
  5. Lai TP
  6. Phizicky EM
  7. Hopper AK
  (2010 Feb 15). Regulation of tRNA bidirectional nuclear-cytoplasmic trafficking in Saccharomyces cerevisiae. Mol Biol Cell. 21, 639-49.
• 1. Phizicky EM
  2. Alfonzo JD
  (2010 Jan 21). Do all modifications benefit all tRNAs? FEBS Lett. 584, 265-71.
• 1. Quartley E
  2. Alexandrov A
  3. Mikucki M
  4. Buckner FS
  5. Hol WG
  6. DeTitta GT
  7. Phizicky EM
  8. Grayhack EJ
  (2009 Sep 01). Heterologous expression of L. major proteins in S. cerevisiae: a test of solubility, purity, and gene recoding. J Struct Funct Genomics. 10, 233-47.
• 1. Phizicky EM
  (2008 Aug 01). Waste not, want not: a case for tRNA repair. Nat Struct Mol Biol. 15, 783-4.
• 1. Merritt EA
  2. Holmes M
  3. Buckner FS
  4. Van Voorhis WC
  5. Quartly E
  6. Phizicky EM
  7. Lauricella A
  8. Luft J
  9. DeTitta G
  10. Neely H
  11. Zucker F
  12. Hol WG
  (2008 Jun 01). Structure of a Trypanosoma brucei alpha/beta-hydrolase fold protein with unknown function. Acta Crystallogr Sect F Struct Biol Cryst Commun. 64, 474-8.
• 1. Chernyakov I
  2. Whipple JM
  3. Kotelawala L
  4. Grayhack EJ
  5. Phizicky EM
  (2008 May 15). Degradation of several hypomodified mature tRNA species in Saccharomyces cerevisiae is mediated by Met22 and the 5'-3' exonucleases Rat1 and Xrn1. Genes Dev. 22, 1369-80.
• 1. Jackman JE
  2. Phizicky EM
  (2008 Apr 22). Identification of critical residues for G-1 addition and substrate recognition by tRNA(His) guanylyltransferase. Biochemistry. 47, 4817-25.
• 1. Chernyakov I
  2. Baker MA
  3. Grayhack EJ
  4. Phizicky EM
  (2008 Jan 01). Chapter 11. Identification and analysis of tRNAs that are degraded in Saccharomyces cerevisiae due to lack of modifications. Methods Enzymol. 449, 221-37.
• 1. Jackman JE
  2. Grayhack EJ
  3. Phizicky EM
  (2008 Jan 01). The use of Saccharomyces cerevisiae proteomic libraries to identify RNA-modifying proteins. Methods Mol Biol. 488, 383-93.
• 1. Kotelawala L
  2. Grayhack EJ
  3. Phizicky EM
  (2008 Jan 01). Identification of yeast tRNA Um(44) 2'-O-methyltransferase (Trm44) and demonstration of a Trm44 role in sustaining levels of specific tRNA(Ser) species. RNA. 14, 158-69.
• 1. Malkowski MG
  2. Quartley E
  3. Friedman AE
  4. Babulski J
  5. Kon Y
  6. Wolfley J
  7. Said M
  8. Luft JR
  9. Phizicky EM
  10. DeTitta GT
  11. Grayhack EJ
  (2007 Apr 17). Blocking S-adenosylmethionine synthesis in yeast allows selenomethionine incorporation and multiwavelength anomalous dispersion phasing. Proc Natl Acad Sci U S A. 104, 6678-83.
• 1. Wilkinson ML
  2. Crary SM
  3. Jackman JE
  4. Grayhack EJ
  5. Phizicky EM
  (2007 Mar 01). The 2'-O-methyltransferase responsible for modification of yeast tRNA at position 4. RNA. 13, 404-13.
• 1. Jackman JE
  2. Kotelawala L
  3. Grayhack EJ
  4. Phizicky EM
  (2007 Jan 01). Identification and characterization of modification enzymes by biochemical analysis of the proteome. Methods Enzymol. 425, 139-52.
• 1. Phizicky EM
  2. Grayhack EJ
  (2006 Aug 16). Proteome-scale analysis of biochemical activity. Crit Rev Biochem Mol Biol. 41, 315-27.
• 1. Jackman JE
  2. Phizicky EM
  (2006 Jun 06). tRNAHis guanylyltransferase catalyzes a 3'-5' polymerization reaction that is distinct from G-1 addition. Proc Natl Acad Sci U S A. 103, 8640-5.
• 1. Jackman JE
  2. Phizicky EM
  (2006 Jun 01). tRNAHis guanylyltransferase adds G-1 to the 5' end of tRNAHis by recognition of the anticodon, one of several features unexpectedly shared with tRNA synthetases. RNA. 12, 1007-14.
• 1. Alexandrov A
  2. Chernyakov I
  3. Gu W
  4. Hiley SL
  5. Hughes TR
  6. Grayhack EJ
  7. Phizicky EM
  (2006 Jan 06). Rapid tRNA decay can result from lack of nonessential modifications. Mol Cell. 21, 87-96.
• 1. Shull NP
  2. Spinelli SL
  3. Phizicky EM
  (2005 Dec 01). We are writing to express our concerns regarding the recently published article by Kumaran et al. Protein Sci. 14, 3145; author reply 3145.
• 1. Gelperin DM
  2. White MA
  3. Wilkinson ML
  4. Kon Y
  5. Kung LA
  6. Wise KJ
  7. Lopez-Hoyo N
  8. Jiang L
  9. Piccirillo S
  10. Yu H
  11. Gerstein M
  12. Dumont ME
  13. Phizicky EM
  14. Snyder M
  15. Grayhack EJ
  (2005 Dec 01). Biochemical and genetic analysis of the yeast proteome with a movable ORF collection. Genes Dev. 19, 2816-26.
• 1. Gu W
  2. Hurto RL
  3. Hopper AK
  4. Grayhack EJ
  5. Phizicky EM
  (2005 Sep 01). Depletion of Saccharomyces cerevisiae tRNA(His) guanylyltransferase Thg1p leads to uncharged tRNAHis with additional m(5)C. Mol Cell Biol. 25, 8191-201.
• 1. Phizicky EM
  (2005 Aug 09). Have tRNA, will travel. Proc Natl Acad Sci U S A. 102, 11127-8.
• 1. Cartlidge RA
  2. Knebel A
  3. Peggie M
  4. Alexandrov A
  5. Phizicky EM
  6. Cohen P
  (2005 May 04). The tRNA methylase METTL1 is phosphorylated and inactivated by PKB and RSK in vitro and in cells. EMBO J. 24, 1696-705.
• 1. Alexandrov A
  2. Grayhack EJ
  3. Phizicky EM
  (2005 May 01). tRNA m7G methyltransferase Trm8p/Trm82p: evidence linking activity to a growth phenotype and implicating Trm82p in maintaining levels of active Trm8p. RNA. 11, 821-30.
• 1. Shull NP
  2. Spinelli SL
  3. Phizicky EM
  (2005 Jan 01). A highly specific phosphatase that acts on ADP-ribose 1''-phosphate, a metabolite of tRNA splicing in Saccharomyces cerevisiae. Nucleic Acids Res. 33, 650-60.
• 1. Steiger MA
  2. Jackman JE
  3. Phizicky EM
  (2005 Jan 01). Analysis of 2'-phosphotransferase (Tpt1p) from Saccharomyces cerevisiae: evidence for a conserved two-step reaction mechanism. RNA. 11, 99-106.
• 1. Alexandrov A
  2. Vignali M
  3. LaCount DJ
  4. Quartley E
  5. de Vries C
  6. De Rosa D
  7. Babulski J
  8. Mitchell SF
  9. Schoenfeld LW
  10. Fields S
  11. Hol WG
  12. Dumont ME
  13. Phizicky EM
  14. Grayhack EJ
  (2004 Sep 01). A facile method for high-throughput co-expression of protein pairs. Mol Cell Proteomics. 3, 934-8.
• 1. Xing F
  2. Hiley SL
  3. Hughes TR
  4. Phizicky EM
  (2004 Apr 23). The specificities of four yeast dihydrouridine synthases for cytoplasmic tRNAs. J Biol Chem. 279, 17850-60.
• 1. Gu W
  2. Jackman JE
  3. Lohan AJ
  4. Gray MW
  5. Phizicky EM
  (2003 Dec 01). tRNAHis maturation: an essential yeast protein catalyzes addition of a guanine nucleotide to the 5' end of tRNAHis. Genes Dev. 17, 2889-901.
• 1. Jackman JE
  2. Montange RK
  3. Malik HS
  4. Phizicky EM
  (2003 May 01). Identification of the yeast gene encoding the tRNA m1G methyltransferase responsible for modification at position 9. RNA. 9, 574-85.
• 1. Hopper AK
  2. Phizicky EM
  (2003 Jan 15). tRNA transfers to the limelight. Genes Dev. 17, 162-80.
• 1. Alexandrov A
  2. Martzen MR
  3. Phizicky EM
  (2002 Oct 01). Two proteins that form a complex are required for 7-methylguanosine modification of yeast tRNA. RNA. 8, 1253-66.
• 1. Xing F
  2. Martzen MR
  3. Phizicky EM
  (2002 Mar 01). A conserved family of Saccharomyces cerevisiae synthases effects dihydrouridine modification of tRNA. RNA. 8, 370-81.
• 1. Phizicky EM
  2. Martzen MR
  3. McCraith SM
  4. Spinelli SL
  5. Xing F
  6. Shull NP
  7. Van Slyke C
  8. Montagne RK
  9. Torres FM
  10. Fields S
  11. Grayhack EJ
  (2002 Jan 01). Biochemical genomics approach to map activities to genes. Methods Enzymol. 350, 546-59.
• 1. Steiger MA
  2. Kierzek R
  3. Turner DH
  4. Phizicky EM
  (2001 Nov 20). Substrate recognition by a yeast 2'-phosphotransferase involved in tRNA splicing and by its Escherichia coli homolog. Biochemistry. 40, 14098-105.
• 1. Grayhack EJ
  2. Phizicky EM
  (2001 Feb 01). Genomic analysis of biochemical function. Curr Opin Chem Biol. 5, 34-9.
• 1. Kierzek R
  2. Steiger MA
  3. Spinelli SL
  4. Turner DH
  5. Phizicky EM
  (2000 Oct 04). The chemical synthesis of oligoribonucleotides with selectively placed 2'-O-phosphates. Nucleosides Nucleotides Nucleic Acids. 19, 917-33.
• 1. Polevoda B
  2. Martzen MR
  3. Das B
  4. Phizicky EM
  5. Sherman F
  (2000 Jul 07). Cytochrome c methyltransferase, Ctm1p, of yeast. J Biol Chem. 275, 20508-13.
• 1. Martzen MR
  2. McCraith SM
  3. Spinelli SL
  4. Torres FM
  5. Fields S
  6. Grayhack EJ
  7. Phizicky EM
  (1999 Nov 05). A biochemical genomics approach for identifying genes by the activity of their products. Science. 286, 1153-5.
• 1. Spinelli SL
  2. Kierzek R
  3. Turner DH
  4. Phizicky EM
  (1999 Jan 29). Transient ADP-ribosylation of a 2'-phosphate implicated in its removal from ligated tRNA during splicing in yeast. J Biol Chem. 274, 2637-44.
• 1. Spinelli SL
  2. Malik HS
  3. Consaul SA
  4. Phizicky EM
  (1998 Nov 24). A functional homolog of a yeast tRNA splicing enzyme is conserved in higher eukaryotes and in Escherichia coli. Proc Natl Acad Sci U S A. 95, 14136-41.