Current Research Projects
Identification of a Molecular Signature for Barrier Insulators
Barrier insulators function to actively maintain the boundaries between heterochromatin (inactive DNA) and euchromatin (actively transcribed DNA). They are critical for regulation of cell-type specific gene expression in normal development and differentiation. Mutations that disrupt barrier insulator function have been associated with developmental disorders, malignancies, and inherited hemolytic anemias. Barrier insulators are poorly understood in mammalian cells, with much of the available data coming from model organisms.
This project combines genome-wide studies of barrier-associated factors with functional assays to identify a common regulatory signal for cell-type specific, functional, barrier insulators in primary human erythroid cells.
Unbiased identification of barrier insulators on a genome wide scale will provide novel insights into normal erythropoiesis and its perturbation in human disease.
References1. Gallagher et al. J Clin Invest. 2010 Dec;120(12):4453-65.
University of Rochester Medical Center
Center for Pediatric Biomedical Research
601 Elmwood Avenue
Rochester, NY 14642