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Principal Investigator

Yi-Tao Yu, Ph.D. University of Rochester work Box 712 601 Elmwood Ave Rochester NY 14642 office: MC 3-6518 p 585-275-1271 f 585-275-6007 yitao_yu@urmc.rochester.edu

Recent Publications

The following list was auto-generated 1 week ago.

• 1. Huang C
  2. Yu YT
  (2013 Apr 01). Synthesis and labeling of RNA in vitro. Curr Protoc Mol Biol. Chapter 4, Unit4.15.
• 1. Ge J
  2. Yu YT
  (2013 Apr 01). RNA pseudouridylation: new insights into an old modification. Trends Biochem Sci. 38, 210-8.
• 1. Huang C
  2. Wu G
  3. Yu YT
  (2012 Apr 01). Inducing nonsense suppression by targeted pseudouridylation. Nat Protoc. 7, 789-800.
• 1. Wu G
  2. Yu AT
  3. Kantartzis A
  4. Yu YT
  (2011 Sep 29). Functions and mechanisms of spliceosomal small nuclear RNA pseudouridylation. Wiley Interdiscip Rev RNA. 2, 571-81.
• 1. Yu AT
  2. Ge J
  3. Yu YT
  (2011 Sep 01). Pseudouridines in spliceosomal snRNAs. Protein Cell. 2, 712-25.
• 1. Karijolich J
  2. Yu YT
  (2011 Jun 16). Converting nonsense codons into sense codons by targeted pseudouridylation. Nature. 474, 395-8.
• 1. Wu G
  2. Yu AT
  3. Kantartzis A
  4. Yu YT
  (2011 Feb 18). Functions and mechanisms of spliceosomal small nuclear RNA pseudouridylation. Wiley Interdisciplinary Reviews (WIREs) RNA. 2, 571-581.
• 1. Wu G
  2. Xiao M
  3. Yang C
  4. Yu YT
  (2011 Jan 05). U2 snRNA is inducibly pseudouridylated at novel sites by Pus7p and snR81 RNP. EMBO J. 30, 79-89.
• 1. Huang C
  2. Karijolich J
  3. Yu YT
  (2011 Jan 01). Post-transcriptional modification of RNAs by artificial Box H/ACA and Box C/D RNPs. Methods Mol Biol. 718, 227-44.
• 1. Yu AT
  2. Ge J
  3. Yu YT
  (2011). The role of alkanethiol spacers in a metal surface-based label-free DNA detection system. Protein & Cell. In press.
• 1. Huang C
  2. Yu YT
  (2010 Sep 01). Targeted 2'-O methylation at a nucleotide within the pseudoknot of telomerase RNA reduces telomerase activity in vivo. Mol Cell Biol. 30, 4368-78.
• 1. Chen C
  2. Zhao X
  3. Kierzek R
  4. Yu YT
  (2010 Sep 01). A flexible RNA backbone within the polypyrimidine tract is required for U2AF65 binding and pre-mRNA splicing in vivo. Mol Cell Biol. 30, 4108-19.
• 1. Karijolich J
  2. Yu YT
  (2010 Jun 28). Spliceosomal snRNA modifications and their function. RNA Biol. 7, 192-204.
• 1. Ge J
  2. Liu H
  3. Yu YT
  (2010 May 01). Regulation of pre-mRNA splicing in Xenopus oocytes by targeted 2'-O-methylation. RNA. 16, 1078-85.
• 1. Karijolich J
  2. Kantartzis A
  3. Yu YT
  (2010 Jan 01). Quantitative analysis of RNA modifications. Methods Mol Biol. 629, 21-32.
• 1. Karijolich J
  2. Kantartzis A
  3. Yu YT
  (2010 Jan 01). RNA modifications: a mechanism that modulates gene expression. Methods Mol Biol. 629, 1-19.
• 1. Xiao M
  2. Yang C
  3. Schattner P
  4. Yu YT
  (2009 Jan 01). Functionality and substrate specificity of human box H/ACA guide RNAs. RNA. 15, 176-86.
• 1. Karijolich J
  2. Yu YT
  (2008 Jul 01). Insight into the Protein Components of the Box H/ACA RNP. Curr Proteomics. 5, 129-137.
• 1. Zhao X
  2. Yu YT
  (2008 Jan 01). Targeted pre-mRNA modification for gene silencing and regulation. Nat Methods. 5, 95-100.
• 1. Dai Q
  2. Fong R
  3. Saikia M
  4. Stephenson D
  5. Yu YT
  6. Pan T
  7. Piccirilli JA
  (2007 Jan 01). Identification of recognition residues for ligation-based detection and quantitation of pseudouridine and N6-methyladenosine. Nucleic Acids Res. 35, 6322-9.
• 1. Karijolich J
  2. Stephenson D
  3. Yu YT
  (2007 Jan 01). Biochemical purification of box H/ACA RNPs involved in pseudouridylation. Methods Enzymol. 425, 241-62.
• 1. Zhao X
  2. Yu YT
  (2007 Jan 01). Incorporation of 5-fluorouracil into U2 snRNA blocks pseudouridylation and pre-mRNA splicing in vivo. Nucleic Acids Res. 35, 550-8.
• 1. Yu YT
  (2006 Feb 01). The most complex pseudouridylase. Structure. 14, 167-8.
• 1. Moon KH
  2. Zhao X
  3. Yu YT
  (2006 Jan 01). Pre-mRNA splicing in the nuclei of Xenopus oocytes. Methods Mol Biol. 322, 149-63.
• 1. Ma X
  2. Yang C
  3. Alexandrov A
  4. Grayhack EJ
  5. Behm-Ansmant I
  6. Yu YT
  (2005 Jul 06). Pseudouridylation of yeast U2 snRNA is catalyzed by either an RNA-guided or RNA-independent mechanism. EMBO J. 24, 2403-13.
• 1. Yang C
  2. McPheeters DS
  3. Yu YT
  (2005 Feb 25). Psi35 in the branch site recognition region of U2 small nuclear RNA is important for pre-mRNA splicing in Saccharomyces cerevisiae. J Biol Chem. 280, 6655-62.
• 1. Zhao X
  2. Yu YT
  (2004 Jun 01). Detection and quantitation of RNA base modifications. RNA. 10, 996-1002.
• 1. Zhao X
  2. Yu YT
  (2004 Apr 01). Pseudouridines in and near the branch site recognition region of U2 snRNA are required for snRNP biogenesis and pre-mRNA splicing in Xenopus oocytes. RNA. 10, 681-90.
• 1. Behm-Ansmant I
  2. Urban A
  3. Ma X
  4. Yu YT
  5. Motorin Y
  6. Branlant C
  (2003 Nov 01). The Saccharomyces cerevisiae U2 snRNA:pseudouridine-synthase Pus7p is a novel multisite-multisubstrate RNA:Psi-synthase also acting on tRNAs. RNA. 9, 1371-82.
• 1. Ma X
  2. Zhao X
  3. Yu YT
  (2003 Apr 15). Pseudouridylation (Psi) of U2 snRNA in S. cerevisiae is catalyzed by an RNA-independent mechanism. EMBO J. 22, 1889-97.
• 1. Zhao X
  2. Li ZH
  3. Terns RM
  4. Terns MP
  5. Yu YT
  (2002 Dec 01). An H/ACA guide RNA directs U2 pseudouridylation at two different sites in the branchpoint recognition region in Xenopus oocytes. RNA. 8, 1515-25.
• 1. Yu YT
  2. Shu MD
  3. Narayanan A
  4. Terns RM
  5. Terns MP
  6. Steitz JA
  (2001 Mar 19). Internal modification of U2 small nuclear (sn)RNA occurs in nucleoli of Xenopus oocytes. J Cell Biol. 152, 1279-88.
• 1. Yu YT
  (2000 Jan 01). Site-specific 4-thiouridine incorporation into RNA molecules. Methods Enzymol. 318, 71-88.
• 1. Yu YT
  (1999 May 01). Construction of 4-thiouridine site-specifically substituted RNAs for cross-linking studies. Methods. 18, 13-21.
• 1. Yu YT
  2. Shu MD
  3. Steitz JA
  (1998 Oct 01). Modifications of U2 snRNA are required for snRNP assembly and pre-mRNA splicing. EMBO J. 17, 5783-95.
• 1. Lapham J
  2. Yu YT
  3. Shu MD
  4. Steitz JA
  5. Crothers DM
  (1997 Sep 01). The position of site-directed cleavage of RNA using RNase H and 2'-O-methyl oligonucleotides is dependent on the enzyme source. RNA. 3, 950-1.
• 1. Yu YT
  2. Steitz JA
  (1997 Jul 01). A new strategy for introducing photoactivatable 4-thiouridine ((4S)U) into specific positions in a long RNA molecule. RNA. 3, 807-10.
• 1. Yu YT
  2. Steitz JA
  (1997 Jun 10). Site-specific crosslinking of mammalian U11 and u6atac to the 5' splice site of an AT-AC intron. Proc Natl Acad Sci U S A. 94, 6030-5.
• 1. Yu YT
  2. Shu MD
  3. Steitz JA
  (1997 Mar 01). A new method for detecting sites of 2'-O-methylation in RNA molecules. RNA. 3, 324-31.
• 1. Yu YT
  2. Tarn WY
  3. Yario TA
  4. Steitz JA
  (1996 Dec 15). More Sm snRNAs from vertebrate cells. Exp Cell Res. 229, 276-81.
• 1. Denker JA
  2. Maroney PA
  3. Yu YT
  4. Kanost RA
  5. Nilsen TW
  (1996 Aug 01). Multiple requirements for nematode spliced leader RNP function in trans-splicing. RNA. 2, 746-55.
• 1. Maroney PA
  2. Yu YT
  3. Jankowska M
  4. Nilsen TW
  (1996 Aug 01). Direct analysis of nematode cis- and trans-spliceosomes: a functional role for U5 snRNA in spliced leader addition trans-splicing and the identification of novel Sm snRNPs. RNA. 2, 735-45.
• 1. Yu YT
  2. Maroney PA
  3. Darzynkiwicz E
  4. Nilsen TW
  (1995 Mar 01). U6 snRNA function in nuclear pre-mRNA splicing: a phosphorothioate interference analysis of the U6 phosphate backbone. RNA. 1, 46-54.
• 1. Yu YT
  2. Maroney PA
  3. Nilsen TW
  (1993 Dec 17). Functional reconstitution of U6 snRNA in nematode cis- and trans-splicing: U6 can serve as both a branch acceptor and a 5' exon. Cell. 75, 1049-59.
• 1. Hannon GJ
  2. Maroney PA
  3. Yu YT
  4. Hannon GE
  5. Nilsen TW
  (1992 Dec 11). Interaction of U6 snRNA with a sequence required for function of the nematode SL RNA in trans-splicing. Science. 258, 1775-80.
• 1. Yu YT
  2. Nilsen TW
  (1992 May 05). Sequence requirements for maturation of the 5' terminus of human 18 S rRNA in vitro. J Biol Chem. 267, 9264-8.