Technology ID: 6-1946
2'-O-methylation at U809 reduces telomerase activity resulting in telomere shortening, whereas 2'-O-methylation at A804 or A805 leads to moderate telomere lengthening. Researchers at the University of Rochester have shown that the use of 2'-O-methylation can be accomplished in vivo and have an effect on telomerase activity.
Technology ID: 6-1446
This technology describes a novel approach to silencing gene expression by interfering with the processing of its mRNA transcript. This method is advantageous over more traditional methods such as iRNA, because it can be used to silence specific alternatively spliced variants of the gene. This is particularly advantageous relative to the development of human therapeutics as it is believed 50% of human transcripts are alternatively spliced.
- Synthesis and labeling of RNA in vitro. Curr Protoc Mol Biol. Chapter 4, Unit4.15. (2013 Apr 01).
- RNA pseudouridylation: new insights into an old modification. Trends Biochem Sci. 38, 210-8. (2013 Apr 01).
- Inducing nonsense suppression by targeted pseudouridylation. Nat Protoc. 7, 789-800. (2012 Apr 01).