Supplemental Data for Sim & Keyoung et al., 2006

Neurocytoma is a Tumor of Adult Neuronal Progenitor Cells

Fraser J. Sim1*; H. Michael Keyoung2*; James E. Goldman3; D.G. Kim4; Sun-Ha Paek2,4; Steven A. Goldman1,2

1Dept. of Neurology, University of Rochester Medical Center, Rochester, NY; 2Dept. of Neurology, Weill Medical College of Cornell University, NY, NY; 3Dept. of Pathology, Columbia University Medical School, NYC, NY; 4Dept. of Neurosurgery, Seoul National University Hospital, Seoul, South Korea.

*The first 2 authors contributed equally to this study.

Supplemental Figure 1
Functional classification of genes over expressed in Neurocytoma relative to
adult VZ and/or adult nestin-sorted progenitors
Click any region on the venn diagrams above to view details of the genes and expression patterns in Neurocytoma.

Supplemental Table 1

Genes significantly enriched in Neurocytoma relative to normal adult VZ tissue

Affymetrix U133A microarray analysis was performed on total RNA extracted from three neurocytoma tumor resections and the resulting expression profiles compared against normal adult human ventricular zone tissue (adult VZ). Affymetrix Probe sets significantly expressed in neurocytoma were identified by two-way t-test against both adult VZ. These probe sets were annotated to 835 genes. This table indicates the ratio of neurocytoma gene expression relative to that of adult VZ; expression ratios are shown with standard errors. All genes reached statistical significance using a 10% false-discovery rate. Only probe sets flagged as 'present' by MAS5 in all three neurocytoma samples were used in the analysis.

Supplemental Table 2

Genes significantly enriched in Neurocytoma relative to nestin-sorted VZ progenitors from adult human brain

Affymetrix U133A microarray analysis was performed on total RNA extracted from three neurocytoma tumor resections and the resulting expression profiles compared against nestin-sorted progenitors from normal adult human ventricular zone tissue (NSP). Affymetrix Probe sets significantly expressed in neurocytoma were identified by two-way t-test against adult NSPs. These probe sets were annotated to 784 genes. This table indicates the ratio of neurocytoma gene expression relative to that of adult NSPs; expression ratios are shown with standard errors. All genes reached statistical significance using a 10% false-discovery rate. Only probe sets flagged as 'present' by MAS5 in all three neurocytoma samples were used in the analysis.