Principal Investigator

Toru Takimoto, D.V.M., Ph.D. University of Rochester work Box 672 601 Elmwood Ave Rochester NY 14642 p 585-273-2856 f 585-273-9573

Contact

Takimoto Lab University of Rochester work 601 Elmwood Ave Rochester NY 14642 p 585-273-2894 f 585-273-9573

Affiliations

Welcome to the Takimoto Lab

Paramyxovirus Assembly

Efficient assembly and release of progeny virions from infected cells are major determinants of viral pathogenicity. Paramyxoviruses are assembled at the plasma membrane budding sites after synthesis of all the structural components in the cytoplasm. Although viral ribonuclocapsid (vRNP) is an essential component of infectious virions, the process of vRNP translocation to assembly sites is poorly understood. To unveil the cellular machinery involved in virus assembly, we rescued a recombinant Sendai virus rSeV-LeGFP, which expresses L protein fused to enhanced green fluorescent protein and characterize the trafficking of viral nucleocapsid to the site of assembly in live cells.

Role of Influenza Polymerase in Host Adaptation

We participate in the virology research team of the New York Influenza Center of Excellence (NYICE), which is part of the NIH Centers of Excellence for Influenza Research and Surveillance (CEIRS) network. Our focus is on the influenza virus RNA polymerase, and understanding how it contributes to host adaptation. We characterize the polymerase activities of avian and human strains, and identify residues and subunits of the polymerase complex important for the enhanced activity in mammalian hosts in vitro and in vivo. We also investigate the additional functions of viral polymerase in host cell shutdown and their role in viral pathogenicity.

Our focus is on the influenza virus RNA polymerase, and understanding how it contributes to host adaptation. We characterize the polymerase activities of avian and human strains, and identify residues and subunits of the polymerase complex important for the enhanced activity in mammalian hosts in vitro and in vivo.

Current Research Projects