Available Resources
Table 1: Xenopus Lymphocyte surface markers detectable with available mAbs
| Xenopus markers (mAbs) | Expression Pattern |
|---|---|
| CD8 (AM22, F17) |
Larval and adult thymocytes (70-80%) and T-cells (about 20% of splenocytes). All lymphoid tumor lines |
| CTX (X71, 1S9.2) |
Larval and adult thymocytes (60-70%)no consistent expression in periphery. All lymphoid tumor lines and gut epithelial tissue. |
| XT1 (XT1) |
Most, but not all, larval and adult T-cells; earliest marker of thymocytes. All lymphoid tumor lines |
| MHC class I (TB17) |
Ubiquitous in adult; all lymphopoietic lineages. Not expressed until metamorphosis |
| MHC class II (AM20, 14A2) |
Thymocytes, B and T-cells (99% of spleen lymphocytes), only B-cells in larvae |
| CD5 (2B1) |
Thymocytes (>95%), T-cells and some PMA-activated IgM+ B cells. All lymphoid tumor lines |
| NK-like (1F8) |
Non-B and non-T, peripheral lymphoid cells. |
| RC47 | Leukocyte lineage from very early stage. Thymic cortex and medulla (>90% of total thymocytes) |
| IgM (10A9) |
Larval and adult B cells. |
| IgY (11D5) |
Some larval and adult B cells. |
| IgX (410D9) |
Some larval and adult B cells, especially in the gut. |
Table 2: Some available cDNA encoding immunologically-relevant X. laevis genes
| Immune System Characteristics | Larva (Ancestral-like system) Adult (Mammalian-like system) |
|---|---|
| CTX | Thymus, all lymphoid tumor lines and gut epithelial tissue. |
| TCR α, β, γ | thymus, circulating T-cells |
| BCR μ, γ, ξ, L | B cells |
| MHC class I | ubiquitous in adult |
| Non-classical class Ib | more restricted tissue distribution for some of them MHC class II |
| MHC class II | thymus, splenic lymphocytes |
| Rag 1 & 2 | mainly thymus, at lower level in bone marrow, kidney, spleen and liver |
| TdT | mainly thymus of old larvae and adult, less in spleen |
| Ikaros | mainly thymus |
| GATA III | thymus and T-cells |
| LMP7 | Thymus, spleen |
| Hsps (gp96, hsp70) | Ubiquitousely expressed, |
Table 3: Currently available X. laevis MHC-defined stains and clones
| Name, (MHC gentotype) | |
|---|---|
| Partially inbred, MHC homozygous strains | F, J |
| Isogenetic leavis/gilli (LG) clones with identical heterozygous (a/c) MHC genotype | LG-6, LG-7, LG-15 |
| MHC-disparate LG isogenetic clones | LG-3 (b/d), LG-5 (b/c) |
Table 4: Available cloned thymic tumor cell lines
| Name of Tumor Cell Line | BB7 | ff-2 | 15/0 |
|---|---|---|---|
| Genetic background of initial tumor-bearing host | Partially inbred MHC homozygous ff strain |
LG-15 (MHC a/c) isogenetic clone | |
| T-cell surface markers (CD8, CD5, pan T-cell) | +++ | +++ | +++ |
| Ig mRNA | - | + | + |
| Ig protein | - | - | - |
| MHC class I mRNA and protein | - | + | - |
| MHC class II mRNA and protein | - | - | - |
| Tumorigenicity in syngeneic | |||
| Larvae | - | + | ++ |
| Adult | |||
List of available genomic and cDNA libraries
| X. laevis cDNA libraries |
|
|---|---|
| Old libraries in λZAPII vector | |
| ST: | Outbred adult spleen + thymus (constructed by L. Du Pasquier, Basel Switzerland)) |
| LG7Ad: | Adult from the clone LG7 immunized with DNP-KLH + CFA (constructed L. Du Pasquier) |
| LG7 Tadp: | LG7 pre-metamorphic larvae immunized DNP-KLH + alum (constructed by M. Wilson, Jakson MS) |
| SMART cDNA libraries (λZAP-express, constructed by S. Guselnikov and A. Taranin, Novosibirsk, Russia) |
|
| T: | Outbred larval spleen + thymus + liver. 7x105 independent clones, titer 6x109 |
| S: | Outbred adult spleen. 2x106 independent clones, titer 1x109 |
| NK: | NK cells, 1F8+ purified from outbred adult splenocytes. 3x106 independent clones, titer 1x1010 |
| T: | CD8 NK/T cells (AM22+/1F8+) purified from outbred splenocytes. 5x105 independent clones, titer 4x109 |
| Arrayed EST cDNA libraries (constructed by Express Genomics, Inc) |
|
| Outbred adult thymus | |
| Outbred adult splenocytes activated for 4 days with PHA | |
| X. tropicalis cDNA libraries (λZAP-express) |
|
| Trop: | Adults spleen + thymus + intestine. Titer 5x1010 |
| Primer | Sequence (5'-3' Forward/Reverse) |
TM (°C) |
Size (bp) |
|---|---|---|---|
| Adult β-globin | GACAGCACATGATCGTCAGC CTTCATGGAAGTGGCGAGCC |
58 | 351 |
| AID | TCACGACCCCCATAGGAACTAC TTAGGAGACTTTGCCTCAAGG |
58 | 450 |
| Arginase 1 | CTTACAATGAGTAGCCAAGG GCAGAGAAAATGAGGATTCG |
60 | 1200 |
| β-actin | GGTGTCATGGTTGGAATGG TGTGGGTTACACCATCACCTG |
57 | 359 |
| β2-M consensus | TGACGGTGAATCCTGGAGAC CGATAGCCGTGACAATGAGC |
57 | 800 |
| C3 | ACCGACATTGACACCACCATTAC CTGCTCCTGTTGTGATTCCTCC |
57 | 493 |
| CD4 | TCCATCTCTGACATCCCCTC TCACCAGACACACGTCCATT |
60 | 895 |
| CD8 α | AAGCCACCTACGACTACCACCAA CCGTTCTTCTCAGTCTCAGGCAC |
59 | 247 |
| CD8 β | TCATCATCTCTTTCTGGGGC AATTCAGTGGGTGCTTCCTG |
60 | 604 |
| CTX | GCAGCAGCGGTAATCGGAG CTCAGCATGGTCATGGAATTG |
58 | 220 |
| Chytrid | AGCCAAGAGATCCGTTGTCAAA CCTTGATATAATACAGTGTGCCATATC |
60 | 250 |
| EF-1α | GAGGGTAGTCTGAGAAGCTCTCCACG CCTGAATCACCCAGGCCAGATTGGTG |
56 | 250 |
| GAPDH | ACCCCTTCATCGACTTGGAC GGAGCCAGACAGTTGTAGTC |
55 | 300 |
| Gp96 | GCGTTGGTTTCTATTCTGCCTTC TGTCTCAGTCTTGCTGCTCCACAC |
56 | 400 |
| HSP72 | AGACTGATCGGGAGAAAGTTC CTTTTCCACAGGTTCCAAGGTACCC |
58 | 747 |
| HSC73 | TGGAGTAGAAAGGAATGTACTGATC AACTTCATTGCATTTCTCCAG |
58 | 1 173 |
| IFNγ | CTGAGGAAATACTTTAACTCCATTGACC TTGTAACATCTCCCACCTGTATTGTC |
60 | 300 |
| Ig γ chain, constant | ATCCAGCAGCCAACTCACCATC AAGGTGGCAGCAAGGTGATTG |
||
| Ig Y | ATGGAGGAGCCAACTCACCATC AAGGTGGCAGCAAGGTGATTG |
58 | 720 |
| Ig M | TCTTCTCTTGTAAGGAACCCG GCAATGCCAAACACCTGG |
58 | 350 |
| IL-1β | AACAGAAGATGGCCAAGACTC ATGCAACCGATTCAAAGC |
60 | 150 |
| LMPX | TATCAGTGTCTTCCGCCTCC CAGCCGTCCTCCTTCATGTG |
56-64 | 300 |
| MAF | TCTTCTTCAGCCTCCCAAGTGAC GCTTTCAGTTCTATTACCTCCCTGC |
57 | 350 |
| MHC class Iα f line |
GAGTTCTTTGCCCTGGATACA CACAATAAGTTTCTCCTTCAGG |
61 | 400 |
| MHC class Iα j line |
GAGTTCATAGCGCTGGATACA CCTCACTGGGTGTAATCTCAG |
61 | 400 |
| MHC II α chain |
TCAAAGTCAGTGGTTTGGACG GTGTAAATATCATGTTCATTTG |
61 | 390 |
| MHC II β chain |
ACGGCACCGACAATGTCAGG TTAATGGGTGTCTCCAGCATC |
61 | 450 |
| MX1 | AGGAGTGGTCAACAGGAGCC TGTTCCGCCGCTGTTCCTCT |
68 | 829 |
| MX2 | GGAACGCCGCACTTGCAGAA CGATTAATCCTGGCACCTCC |
68 | 274 |
| Rag-1 | GCGCCAAGAATCTGTGTCACT GTTCTGTTTCATGGTTGTCTACCA |
58 | 350 |
| TCR α | TTCAGGGACCTCTGGTTGCTTC ACACATCAGCCTCAATCCATCC |
60 | |
| TCR V β 1 | CACCCAGGAGCCAAGATC | 56 | 700 |
| TCR V β 3 | TGACGGTGAATCCTGGAGAC | 58 | 700 |
| TCR V β 6 | GTCAGCTGACATTCCTTGTAATC | 58 | 700 |
| TCR V β 8 | GACCAAAAGTTCTTAGCG | 50 | 700 |
| TCR β constant | CGATAGCCGTGACAATGAGC | 50-58 | 700 |
| TCR γ | AGAAGTTCTCAAGCAGGCAG AAGTCAGCTGAAGAGGATCC |
60 | 328 |
| TNFα | GCTCAAGGATAACTCCATCG AACCAAGTGGCACCTGAATG |
59 | 203 |
| XNC 2 | GGTATCAAACCGTATCTATGGTG TCTCTATATACTCCTTACTATCAG |
59 | 450 |
| XNC 6 | CTTTGCATGATCGTAATG CTGCATATCTAGATGGCTTG |
52 | 386 |
| XNC 7 | CCGATGTGAGCCTTTGTTTCAG GTGCACTCATTTTCCATGAATAGT |
59 | 380 |
| XNC 11 | CGATGACTTCTTCACCGACC CCTTGCTCCACTCTGGTATG |
58 | 350 |
| Primer | Sequence (5'-3' Forward/Reverse) |
TM (°C) |
Size (bp) |
|---|---|---|---|
| DNA polymerase II | ACGAGCCCGACGAAGACTACA TGGTGGTCCTCAGCATCCT |
56 | 378 |
| Early gene | ATGATCCAAGCCTACCTGTGC AAATGTCCTAATCTATACACC |
52 | 450 |
| IE-ICP18 forward | ATGATCCAAGCCTACCTGTGC AAATGTCCTAATCTATACACC |
52 | 450 |
| Intermediate gene | TGCGCGACCGTCAAGCCTATTAC CTGGGAGTTCTGAGGAAGCATTC |
65 | 500 |
| Late gene | TGTGGGCCAGTCCCCCGTGCA AGAGTAGGCGCAGTAGTCGACGACG |
72 | 250 |
| Major capsid protein | GTCTCTGGAGAAGAAGAA GACTTGGCCACTTATGAC |
52 | 525 |
| 53R | ATCAGTCACAGGTCTTTAGCATAG TCTGCTTGATCTCGGGACACAATG |
56 | 441 |
Ordering Info
Please click here to download the order form.
Reource Quick-Links:
Surface Markers
cDNA encoding
Stains & Clones
Cloned thymic tumor cell lines
genomic and cDNA libraries
Primer sequences for immune genes
Primer sequences for Frog Virus 3 genes
