Bottaro Lab
The Team: Andrea Bottaro, Ph.D., Assistant Professor of Medicine
Igor Kuzin, M.D., Ph.D., Research Instructor
Andrea Bottaro, PhD.
Dr. Bottaro's research interests include B cell activation, the molecular biology of antibody gene expression and reconbination, immunoglobulin class switching and trangenic/knock-out mouse models.
Funding
Dr. Bottaro receives funding through the NIH.
Publications
His most recent publications include:
Kuzin II, Ugine GD, Barth RK, Shultz LD, Nahm MH, Young FM, Bottaro A.A new murine model of humoral immuno-deficiency specifically affects classswitching to T-independent antigens. Eur J Immunol. 2004, 34(7):1807-16.
Filatov AV, Shmigol IB, Kuzin II, Sharonov GV, Feofanov AV.Resistance of cellular membrane antigens to solubilization with Triton X-100 as amarker of their association with lipid rafts-analysis by flow cytometry. J Immunol Methods. 2003, 278(1-2):211-9.
Kuzin II, Snyder JE, Ugine GD, Wu D, Lee S, Bushnell T Jr, Insel RA, Young FM,Bottaro A. Tetracyclines inhibit activated B cell function. Int Immunol. 2001 (7):921-31
Kuzin I, Ugine G, Wu D, Young F, Chen J, Bottaro A. Normal isotype switching in B cells lacking the I? exon splice donor site: evidence for multiple I?-like transcripts. J Immunol 164, 1451-1457, 2000
Borghesani PR, Alt FW, Bottaro A, Davidson L, Aksoy S., Rathbun GA, Roberts TM, Swat W, Segal RA, Gu Y. Abnormal development of Purkinje cells and lymphocytes in Atm mutant mice. Proc Natl Acad Sci USA 97, 3336-3341, 2000.
Kuzin, I, Snyder J, Ugine G, Wu D, Lee S, Bushnell T, Insel R, Young F, Bottaro A. Tetracyclines inhibit activated B cell function, Int. Immunol. 12, 921-931, 2001.
For a complete list of Dr. Bottaro's publications, use the following link: publications.
Igor Kuzin, M.D., Ph.D.
Dr. Kuzin is currently working on two major research projects. The goal of the first one is to elucidate the mechanisms that regulate expression and recombination of immunoglobulin heavy chain (IgH) genes. Specifically, he and his are interested in the role of enhancer specificity in the IgH locus regulation. There are several enhancer elements within IgH locus. One of the best characterized is intronic Em enhancer, which plays a role in regulation of gene transcription, VDJ recombination, somatic hypermutation, and immunoglobulin class switching. In their studies, to address the role of enhancer specificity in the regulation of IgH locus activity, they have created transgenic mice bearing either a deletion of the Em core element or replacement of the same element with the strong, ubiquitous SV40 transcriptional enhancer. The phenotype of these transgenic mice is currently under investigation.
The goal of the second project is to develop an artificial in vitro system for the induction of antibody immune response by cultivated human lymphocytes. They use specially designed bioreactors in which human lymphocytes isolated from donor tonsils are propagated, and explore various conditions that would be optimal for the induction of primary and secondary antibody immune responses to a variety of antigens. These studies have potential clinical application.
Funding
Dr. Kuzin receives his funding through the NIH.
Publications
Dr. Kuzin's most recent publications include:
Kuzin II, Ugine GD, Barth RK, Shultz LD, Nahm MH, Young FM, Bottaro A.A new murine model of humoral immuno-deficiency specifically affects classswitching to T-independent antigens. Eur J Immunol. 2004, 34(7):1807-16.
Filatov AV, Shmigol IB, Kuzin II, Sharonov GV, Feofanov AV.Resistance of cellular membrane antigens to solubilization with Triton X-100 as amarker of their association with lipid rafts-analysis by flow cytometry. J Immunol Methods. 2003, 278(1-2):211-9.
Kuzin II, Snyder JE, Ugine GD, Wu D, Lee S, Bushnell T Jr, Insel RA, Young FM,Bottaro A. Tetracyclines inhibit activated B cell function. Int Immunol. 2001 (7):921-31
Kuzin II, Ugine GD, Wu D, Young F, Chen J, Bottaro A. Normal isotype switching in B cells lacking the I mu exon splice donor site:evidence for multiple I mu-like germline transcripts. J Immunol. 2000, 164(3):1451-7
For a complete list of Dr. Kuzin's publications, use the following link: publications:
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