Keith Nehrke NIH 1 R01GM087483 Nehrke PI C. Elegans and Mitchondrial K+ Channels 01/01/10-12/31/13 NIH R01NS64945 Nehrke PI Neuroprotection by a Nuclear Carbonic Anhydrase in C. Elegans 09/01/10-05//31/15 NSF IOS-0919848 Nehrke PI Integrated Calcium and pH Signaling in C. Elegans The focus of our laboratory is on integrative physiology, and in particular on understanding how membrane electrolyte flux and pH regulation influences viability and behavior in a whole organism. Our approach has been to use the genetic model C. elegans and novel, fluorescent based methods for measuring cell (or organelle) specific fluctuations in the level of physiologic signaling molecules such as calcium, cAMP, protons, potassium, and reactive oxygen species in live, unrestrained worms. Our goal is to develop a deeper understanding of how cell autonomous signaling processes are integrated to mediate the environmental sensitivity and functional output exhibited by live animals. We believe that this combination of physiology and genetics will allow us to discover unique mechanisms whereby cells communicate information in their native context. The two projects that are currently funded center around defining the functional intersection between calcium and proton signaling during a rhythmic behavior and determining the molecular identity of mitochondrial potassium channels that are essential for ischemic preconditioning.