Instrumentation & Equipment

Multiphoton Imaging & Laser System

Multiphoton microscope system (Olympus Fluoview 1000 AOM-MPM system for DeepSee laser)
Multiphoton laser system (Spectra-Physics MaiTai HP DeepSee Ti:Sa laser)
Optical Table with electronic vibration control (Newport Smart Table ST-58-12, 5’x6’x8”)
Microscope lens for high-resolution in vivo imaging (Olympus 25x, NA 1.05)
Microscope lens for low-magnification imaging (Zeiss C-Apochromat 10x, NA 0.45)
Microscope lens for orientation, electrode placement and optical imaging (Olympus 4x)
Digital wide-field video camera integrated in Multiphoton imaging system

Autocorrelator (PSCOUT HR, Newport)
Near-IR-Spectrometer (PSPECNIR, Newport)
UV-VIS Spectrometer (BW-Tek BTC1112E)
Optoelectronic modulator with linear amplifier (350-80LA E, Conoptics)
Laser-power meter with high power and low power detector (1918-C, Newport)
Collection of lenses, optical fibers, dichroic mirrors and emission filters (Semrock, Chroma, Newport, Thorlabs)
Collection of opto-mechanical components (Linos, Newport, Thorlabs)
Micrometer-scale for calibration of MPM-microscope system (S21024, Summers optical)

External microscope stage for in vivo imaging in small rodents. Note: Small-animals or sample holders can be directly mounted to adjustable M6-mini-breadboard which are integral part of the microscope stage. An M6 screw set is available for all core users.
External microscope stage adapter for in vitro/ex-vivo imaging (e.g. histological slides, cell-culture dishes and acute slice preparations). Note: Transition time between in vivo and in vitro microscope stage less than 1 minute). Cell- or slice-chambers can be directly mounted to the stage adapter using ¼”-20 screws with one inch (25.4 mm) spacing. A ¼”-20 screw-set is available for all core users.
Intracellular amplifier (Neuroprobe 1600, AM Systems)
Micromanipulators system with 2 closed loop manipulators (MP225, Sutter Instruments)
Pulse generator with 2 stimulus isolators (AMPI Master 8/Isoflex)
Picoammeter for polarographic oxygen measurements
Medical Gas (O2, CO2, N2, room-air) with multi-channel flow-meter
16 channel-real time data acquisition system (Digidata 1440/pCLAMP10, Axon Instruments)

For access to the Multiphoton Core, please fill out the request form and call 273-2780, or email Ben Panepento or Dr. Karl Kasischke.