Instrumentation & Equipment

Multiphoton Imaging & Laser System

  • Multiphoton microscope system (Olympus Fluoview 1000 AOM-MPM system for DeepSee laser)
  • Multiphoton laser system (Spectra-Physics MaiTai HP DeepSee Ti:Sa laser)
  • Optical Table with electronic vibration control (Newport Smart Table ST-58-12, 5’x6’x8”)
  • Microscope lens for high-resolution in vivo  imaging (Olympus 25x, NA 1.05)
  • Microscope lens for low-magnification imaging (Zeiss C-Apochromat 10x, NA 0.45)
  • Microscope lens for orientation, electrode placement and optical imaging (Olympus 4x)
  • Digital wide-field video camera integrated in Multiphoton imaging system

Optical, Opto-Electronical, & Opto-Mechanical Equipment

  • Autocorrelator (PSCOUT HR, Newport)
  • Near-IR-Spectrometer (PSPECNIR, Newport)
  • UV-VIS Spectrometer (BW-Tek BTC1112E)
  • Optoelectronic modulator with linear amplifier (350-80LA E, Conoptics)
  • Laser-power meter with high power and low power detector (1918-C, Newport)
  • Collection of lenses, optical fibers, dichroic mirrors and emission filters (Semrock, Chroma, Newport, Thorlabs)
  • Collection of opto-mechanical components (Linos, Newport, Thorlabs)
  • Micrometer-scale for calibration of MPM-microscope system (S21024, Summers optical)

Equipment for Electrophysiology & Systems Physiology

  • External microscope stage for in vivo imaging in small rodents. Note: Small-animals or sample holders can be directly mounted to adjustable M6-mini-breadboard which are integral part of the microscope stage. An M6 screw set is available for all core users.
  • External microscope stage adapter for in vitro/ex-vivo imaging (e.g. histological slides, cell-culture dishes and acute slice preparations). Note: Transition time between in vivo and in vitro microscope stage less than 1 minute). Cell- or slice-chambers can be directly mounted to the stage adapter using ¼”-20 screws with one inch (25.4 mm) spacing. A ¼”-20 screw-set is available for all core users.
  • Intracellular amplifier (Neuroprobe 1600, AM Systems)
  • Micromanipulators system with 2 closed loop manipulators (MP225, Sutter Instruments)
  • Pulse generator with 2 stimulus isolators (AMPI Master 8/Isoflex)
  • Picoammeter for polarographic oxygen measurements
  • Medical Gas (O2, CO2, N2, room-air)  with multi-channel flow-meter
  • 16 channel-real time data acquisition system (Digidata 1440/pCLAMP10, Axon Instruments)

Software for Image & Data Analysis and Instrument Control (PC only)

  • Olympus Fluoview 1000 Software
  • PCLAMP10 (Axon Instruments)
  • Matlab 32bit and 64bit for PC (latest release) with all toolboxes
  • Origin Pro 7.5 (Statistical analysis and Scientific Graphing)
  • Microsoft Visual Studio 2008 Suite (C++ and Visual Basic compiler)
  • ImageJ (Download Instructions)

MP Core Calendar

Users should email Maria Jepson to arrange for scheduling.

Calendar Link

 

Multiphoton Listserv

Listserv

Events

1st Annual Immune Imaging Symposium To Be Held November 7, 2015