Matthew J. Hilton
Ph.D. 2004 University of Houston
Associate Professor of Orthopaedics,
Director of Histology, Biochemistry, and
Molecular Imaging (HBMI) Core, CMSR
Primary Appointment: Department of Orthopaedics
Center Affiliation: Center for Musculoskeletal Research
Most of the bones in the vertebrate skeleton arise from a cartilage template during embryogenesis. This process, known as endochondral ossification, begins with the differentiation of condensed mesenchymal stem cells (MSCs) into chondroprogenitors (immature cartilage cells) and osteoprogenitors (immature bone cells). Both the chondroprogenitor and osteoprogenitor cells undergo a coupled proliferation and differentiation program ultimately leading to the formation of mature cartilage and bone. Various genetic studies have demonstrated that Ihh, Pthrp, BMPs, FGFs, and canonical Wnt signaling pathways are required at multiple stages of normal cartilage and bone development. Deregulation of these signaling circuits during development are a primary cause for a variety of skeletal dysplasias, as well as, age related cartilage and bone pathologies.
A long-term interest of the Hilton lab is to uncover the molecular circuitry regulating lineage commitment, proliferation, and differentiation of MSCs and maturing chondrocytes. My laboratory uses genetic mouse models and primary cell culture techniques coupled with biochemistry to answer questions regarding MSC self-renewal/differentiation, chondrogenesis, and chondrocyte maturation. Recently my lab has generated novel data from a variety of Notch gain and loss-of-function mutant mice demonstrating that Notch signaling pathway suppresses MSC differentiation and plays critical roles in regulating chondrogenesis and chondrocyte maturation. We are currently investigating the exact Notch signaling mechanisms regulating both early and late stages of these processes, as well as, determining how Notch components interact with other known signaling pathways during cartilage development and maintenance. These studies are also being extended to aid in our mechanistic understanding of both fracture repair and osteoarthritis.
Finally, the Hilton lab is continuing to investigate the molecular mechanisms responsible for a developmental bone and cartilage disorder known as Multiple Hereditary Exostoses (MHE). MHE is an autosomal dominant disease caused by mutations in either the Ext1 or Ext2 genes, subunits of the heparan sulphate co-polymerase complex. Affected individuals are diagnosed with cartilaginous bony outgrowths (exostoses) adjacent to the growth plates of endochondral bones, bowing of some bones, and short stature. Although previous studies have shown that defects in Ext1 and Ext2 lead to reduced synthesis and shortened heparan sulphate chains on cell surface proteoglycans, the exact molecular mechanisms underlying this skeletal disease are still unknown. My lab is currently examining various Ext1 conditional mutant mouse models to determine the precise cell lineage and cause of exostosis formation. Additional genetic studies are also aimed at determining the effect that loss of Ext1 function has on specific signaling pathways important during chondrocyte and osteoblast development.
|MSC Self-renewal and Differentiation|
|Growth Plate Cartilage
Development and Maturation
|Mechanisms of Fracture Repair|
|Articular Cartilage Development,
Maintenance, and Osteoarthritis
Mirando AJ*, Liu Z*, Moore T, Lang A, Jesse AM, Kohn A, O’Keefe RJ, Mooney RA, Zuscik MJ, and Hilton MJ. (2013) RBPjκ-dependent Notch signaling is required for articular cartilage and joint maintenance. Arthritis and Rheumatism. Jul 9. doi: 10.1002/art.38076. [Epub ahead of print]
Kohn A, Dong Y, Mirando A, Jesse AM, Honjo T, Zuscik MJ, O’Keefe RJ, and Hilton MJ. (2012). Cartilage-specific RBPJk-dependent and –independent Notch signals regulate cartilage and bone development. Development. 139(6): 1198-212.
Sampson ER, Hilton MJ, Chen D, Schwarz EM, Bukata SV, O’Keefe RJ, Awad H, Puzas JE, Rosier RN*, and Zuscik MJ*. (2011) Teriparatide as a Chondro-regenerative Therapy for Injury-induced Osteoarthritis. Science Translational Medicine, 3(101): 101ra93.
Shu B, Ming Z, Wang B, Xie R, Wang M, Xin H, Hou W, Tang D, Harris SE, O’Keefe RJ, Hilton MJ, Wang W, and Chen D. (2011) BMP2, but not BMP4, is crucial for chondrocyte proliferation and maturation during endochondral bone development. Journal of Cell Science, 124(Pt20): 3428-40.
Dong Y*, Jesse A*, Kohn A, Gunnell LM, Honjo T, Zuscik MJ, O’Keefe RJ, and Hilton MJ. (2010). RBPJk-dependent Notch Signaling Regulates Mesenchymal Progenitor Cell Proliferation and Differentiation During Skeletal Development. Development, 137, 1461-1471.
Gunnell LM, Jonason JH, Loiselle AE, Kohn A, Schwarz EM, O’Keefe RJ*, and Hilton MJ*. (2010). TAK1 Regulates Cartilage and Joint Development via the MAPK and BMP Signaling Pathways. Journal of Bone and Mineral Research. 25(8): 1784-1797.
Hilton MJ*, Tu X*, Bai S, Zhao H, Kobayashi T, Kronenberg HM, Teitelbaum SL, Ross FP, Kopan R, and Long F. (2008). Notch Signaling Maintains Bone Marrow Mesenchymal Progenitors by Suppressing Osteoblast Differentiation. Nature Medicine, 14 (3): 306-14.
Zuscik M, Hilton MJ, Zhang X, Chen D, and O’Keefe RJ. (2008). Regulation of Chondrogenesis and Chondrocyte Differentiation by Stress. Journal of Clinical Investigation, 118(2): 429-38.
Hilton MJ, Tu X, and Long F. (2007). Tamoxifen-inducible gene deletion reveals a distinct cell type associated with trabecular bone, and direct regulation of PTHrP expression and chondrocyte morphology by Ihh in growth region cartilage. Developmental Biology, 308 (1): 93-105.
Hilton MJ, Tu X, Cook J, Hu H, and Long F. (2005). Ihh Controls Cartilage Development by Antagonizing Gli3 but Requires Additional Effectors to Regulate Osteoblast and Vascular Development. Development, 132(19):4339-51.
Hilton MJ*, Hu H*, Tu X, Yu K, Ornitz DM, and Long F. (2005). Sequential Roles of Hedgehog and Wnt Signaling in Osteoblast Development. Development, 132(1): 49-60.
Hilton MJ, Gutierrez L, Martinez D, and Wells DE. (2005). EXT1 Regulates Chondroctye Proliferation and Differentiation During Endochondral Bone Development. Bone, 36(3): 379-86.
Graduate Program Affiliations
Matthew J. Hilton, Ph.D.
University of Rochester
601 Elmwood Ave., Box 665
Rochester, NY 14642
Office: SMD 1-8562
M.D., Ph.D. Research Assistant Professor
|Anthony J. Mirando, M.S.
Lab Tech Associate I
Laboratory Technician IV
Laboratory Technician III
|Timothy Rutkowski Ph.D. student|