ALUMINON STAIN FOR ALUMINUM
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TECHNIQUE: Undecalcified glycol methacrylate embedded sections cut at 4 μm or paraffin sections cut at 5 μm.
SOLUTIONS:
Buffer Solution, pH 5.2
Ammonium acetate ------------------------------------------------------------- 40.0 gm
Ammonium chloride ------------------------------------------------------------ 28.0 gm
Distilled water ------------------------------------------------------------------ 210.0 ml
50% Hydrochloric acid, (6N) -------------------------------------------------- 27.0 ml
Adjust to pH 5.2 with concentrated hydrochloric acid or 28%
ammonium hydroxide. Store in refrigerator at 3-6o C.
*Aluminon Solution
Aluminon ------------------------------------------------------------------------- 0.8 gm
Buffer solution, pH 5.2 --------------------------------------------------------- -- 40.0 ml
Place in a 125 ml flask and place on a hot plate stirrer. Heat to
80-85o C just before use.
*See Step 2 of the Staining Procedure.
1.6M Ammonium Carbonate
Ammonium carbonate ---------------------------------------------------------- 15.4 gm
Distilled water to make a total of ------------------------------------------------ 100.0 ml
Store in a refrigerator at 3-6o C.
Differentiating Solution, pH 7.2
Buffer solution, pH 5.2 --------------------------------------------------------- 22.0 ml
1.6M ammonium carbonate ---------------------------------------------------- 8.0 ml
Fast Green, Stock Solution
Fast green FCF, C.I. 42053 ---------------------------------------------------- 0.2 gm
Distilled water ------------------------------------------------------------------ 100.0 ml
Acetic acid, glacial -------------------------------------------------------------- 0.2 gm
Fast Green, Working Solution
Fast green, stock solution ------------------------------------------------------ 10.0 ml
Distilled water ----------------------------------------------------------------- 35.0 ml
Aluminum ---------------------------------------------------------- red
Background ------------------------------------------------------- varying shades of green
Lillie, R.D.: Histopathologic Technic and Practical Histochemistry, 4th ed., New York, McGraw-Hill, 1976, p. 534.
Ohtsuki, T. et al.: A simplified aluminum stain in paraffin sections of bone from hemodialysis patients. Stain Technol. 64: 55-59, 1989.


Image Examples:
Diagnostic Application: