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FIXATION:        10% buffered neutral formalin.

TECHNIQUE:        Paraffin sections cut at 6-8 µm.

SOLUTIONS:

Cresyl Violet Acetate Solution

Cresyl violet acetate -------------------------------------------------------------        0.02 gm

Distilled water  ---------------------------------------------------------------        ----        100.00 ml

Glacial acetic acid ---------------------------------------------------------------                0.25 ml

1.        Deparaffinize and hydrate slides to distilled water.

2.        Place slides in cresyl violet acetate solution for 5 minutes.

3.        Rinse in three changes of distilled water.

4.        Dehydrate in graded alcohols.

5.        Clear in three or four changes of xylene.

6.        Mount with synthetic resin.

Nissl substance ------------------------------------------------------                purple

Nuclei ---------------------------------------------------------------        purple

Background ---------------------------------------------------------                clear

Some staining methods require differentiation in 95% alcohol until Nissl substance stands out against a clear background.  In the described method the differentiation takes place when the slides are dehydrated in graded alcohols.

Luna, L.G.:  Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology, 2nd ed., New York, McGraw-Hill Book Co., p,. 160, 1960.