FIXATION: 10% buffered neutral formalin.
TECHNIQUE: Paraffin sections cut at 6-8 µm.
SOLUTIONS:
Gallocyanin Solution
Gallocyanin, C.I. 51030 --------------------------------------------------------- 0.15 gm
Chromium potassium sulfate ----------------------------------------------------- 5.00 gm
Distilled water -------------------------------------------------------------------- 100.00 ml
Dissolve the chrom alum in warm water, add gallocyanin, and boil
gently for 10 minutes. Cool and filter. This solution keeps for one
week.
1. Deparaffinize and hydrate to distilled water.
2. Place sections in gallocyanin solution and place in a 37˚ C oven for 6 hours or leave overnight at room temperature.
3. Rinse in two changes of distilled water.
4. Dehydrate in graded alcohols.
5. Clear in three or four changes of xylene.
6. Mount with synthetic resin.
Image Examples:
Diagnostic Application: