FIXATIVES

10% Buffered Neutral Formalin

Formalin, 37-40% ---------------------------------------------------------------        100.0 ml

Distilled water --------------------------------------------------------------------        900.0 ml

Sodium phosphate, monobasic NaH2PO4•H2O) --------------------------------        4.0 gm

Sodium phosphate, dibasic Na2HPO4 -------------------------------------------        6.5 gm

       The best overall fixative for routine use.

10% Formalin-Sodium Acetate

Formalin, 37-40% ----------------------------------------------------------------        100.0 ml

Tap water ------------------------------------------------------------------------        900.0 ml

Sodium acetate NaC2H3O2•3H20 -----------------------------------------------        20.0 gm

       Formalin with sodium acetate is an excellent fixative and medium

       in which to store gross blocks of tissue.

10% Formalin-Alcohol

Formalin, 37-40% ---------------------------------------------------------------        10.0 ml

Alcohol, absolute ----------------------------------------------------------------        90.0 ml

       This fixative is recommended for the preservation of glycogen.

10% Formalin-Calcium

Formalin, 37-40% ---------------------------------------------------------------        10.0 ml

Distilled water -------------------------------------------------------------------        90.0 ml

Calcium chloride, anhydrous -----------------------------------------------------        1.0 gm

       This fixative is especially useful when studies on lipids are to be

       performed.

Zenkers Solution, Modified

Distilled water --------------------------------------------------------------------        300.0 ml

Zinc acetate -----------------------------------------------------------------------        3.0 gm

Potassium dichromate -----------------------------------------------------------        --        7.5 gm

Acetic acid ------------------------------------------------------------------------        15.0 ml

Bouins Solution

Picric acid, saturated aqueous --------------------------------------------------        --        750.0 ml

Formalin, 37-40% ----------------------------------------------------------------        250.0 ml

Acetic acid, glacial ---------------------------------------------------------------        50.0 ml

Carnoys Solution

Alcohol, absolute ----------------------------------------------------------------        60.0 ml

Chloroform ----------------------------------------------------------------------        30.0 ml

Acetic acid, glacial ---------------------------------------------------------------        10.0 ml

       One of the best penetrating and quick acting fixatives known. 

       It gives excellent nuclear fixation with preservation of Nissl

       substance, plasma cells, and glycogen, but it hemolyzes red

       blood cells.

Lillies B-5 Fixative, Modified

Zinc chloride ---------------------------------------------------------------------        6.0 gm

Sodium acetate, anhydrous -----------------------------------------------------        --        2.5 gm

Distilled water --------------------------------------------------------------------        180.0 ml

Formaldehyde, concentrated -----------------------------------------------------        20.0 ml

       Add the formaldehyde just before use.

Zinc Formalin

Formalin, 37% --------------------------------------------------------------------        10.0 ml

Distilled water --------------------------------------------------------------------        100.0 ml

Zinc chloride ----------------------------------------------------------------------        1.0 gm

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