Paul Kingsley, Ph.D.

Paul Kingsley, Ph.D.

Contact Information

University of Rochester Medical Center
School of Medicine and Dentistry
601 Elmwood Ave, Box 703
Rochester, NY 14642

Office: (585) 275-5073

Research Bio

Dr. Kingsley's research interests are:
1) Embryonic Hematopoiesis
2) Erythroid Differentiation
3) Ontogeny of Hematopoietic Progenitors
4) Erythroid Precursor Self Renewal



Research in his laboratory utilizes a variety of approaches to investigate embryonic hematopoiesis. Formation of blood is a critical early event for mammalian embryos, initially occurring in the extraembryonic yolk sac of the gastrulating embryo, then migrating to the fetal liver, and finally homing to the bone marrow at the end of gestation. They are using subtractive hybridization and degenerate PCR to identify gene family members involved in specification, expansion, mobilization and migration of hematopoietic stem cells and progenitors.

An alternate approach they use is genechip technology (Affymetrix), where tens of thousands of gene products are evaluated quantitatively for expression in different regions of the developing embryo. Target tissues include microdissected regions from early murine embryos as well as embryonic cells triple-sorted for known hematopoietic/angiogenic surface markers. The spatial and temporal expression patterns of candidate genes are confirmed by in situ hybridization to both whole embryos and sections of embryonic tissues. The function of candidate molecules are tested using a variety of in vitro and in vivo model systems.

Recent Journal Articles

Showing the 5 most recent journal articles. 44 available »

2014 Apr 15
Niswander LM, Fegan KH, Kingsley PD, McGrath KE, Palis J. "SDF-1 dynamically mediates megakaryocyte niche occupancy and thrombopoiesis at steady-state and following radiation injury." Blood.. 2014 Apr 15; Epub 2014 Apr 15.
2013 Feb 7
Kingsley PD, Greenfest-Allen E, Frame JM, Bushnell TP, Malik J, McGrath KE, Stoeckert CJ, Palis J. "Ontogeny of erythroid gene expression." Blood.. 2013 Feb 7; 121(6):e5-e13. Epub 2012 Dec 12.
2012 Sep 20
Peslak SA, Wenger J, Bemis JC, Kingsley PD, Koniski AD, McGrath KE, Palis J. "EPO-mediated expansion of late-stage erythroid progenitors in the bone marrow initiates recovery from sublethal radiation stress." Blood.. 2012 Sep 20; 120(12):2501-11. Epub 2012 Aug 13.
2011 May 12
Fromm G, Cadiz-Rivera B, de Vries C, Getman M, McGrath KE, Kingsley PD, Fields J, Fiering S, Bulger M. "An embryonic stage-specific enhancer within the murine ?-globin locus mediates domain-wide histone hyperacetylation." Blood.. 2011 May 12; 117(19):5207-14. Epub 2011 Feb 14.
2011 Apr 28
McGrath KE, Frame JM, Fromm GJ, Koniski AD, Kingsley PD, Little J, Bulger M, Palis J. "A transient definitive erythroid lineage with unique regulation of the ?-globin locus in the mammalian embryo." Blood.. 2011 Apr 28; 117(17):4600-8. Epub 2011 Mar 04.

Current Appointments

Research Assistant Professor - Department of Pediatrics, Hematology and Oncology (SMD) - Primary
Research Assistant Professor - Center for Pediatric Biomedical Research

Education

PhD | Developmental Biology | University of Rochester1992
MS | Developmental Biology | University of Rochester1987
BA | Biology | Reed College1982

Post-Doctoral Training & Residency

Cancer Center Fellowship in Hematopoiesis, University of Rochester School of Medicine and Dentistry, Rochester, NY 1994