Paul Kingsley, Ph.D.

Paul Kingsley, Ph.D.

Contact Information

University of Rochester Medical Center
School of Medicine and Dentistry
601 Elmwood Ave, Box 703
Rochester, NY 14642

Office: (585) 275-5073

Research Bio

Dr. Kingsley's research interests are:
1) Embryonic Hematopoiesis
2) Erythroid Differentiation
3) Ontogeny of Hematopoietic Progenitors
4) Erythroid Precursor Self Renewal

Research in his laboratory utilizes a variety of approaches to investigate embryonic hematopoiesis. Formation of blood is a critical early event for mammalian embryos, initially occurring in the extraembryonic yolk sac of the gastrulating embryo, then migrating to the fetal liver, and finally homing to the bone marrow at the end of gestation. They are using subtractive hybridization and degenerate PCR to identify gene family members involved in specification, expansion, mobilization and migration of hematopoietic stem cells and progenitors.

An alternate approach they use is genechip technology (Affymetrix), where tens of thousands of gene products are evaluated quantitatively for expression in different regions of the developing embryo. Target tissues include microdissected regions from early murine embryos as well as embryonic cells triple-sorted for known hematopoietic/angiogenic surface markers. The spatial and temporal expression patterns of candidate genes are confirmed by in situ hybridization to both whole embryos and sections of embryonic tissues. The function of candidate molecules are tested using a variety of in vitro and in vivo model systems.

Recent Journal Articles

Showing the 5 most recent journal articles. 47 available »

2015 Jun 30
McGrath KE, Frame JM, Fegan KH, Bowen JR, Conway SJ, Catherman SC, Kingsley PD, Koniski AD, Palis J. "Distinct Sources of Hematopoietic Progenitors Emerge before HSCs and Provide Functional Blood Cells in the Mammalian Embryo." Cell reports. 2015 Jun 30; 11(12):1892-904. Epub 2015 Jun 18.
2015 Jun 9
Kim AR, Olsen JL, England SJ, Huang YS, Fegan KH, Delgadillo LF, McGrath KE, Kingsley PD, Waugh RE, Palis J. "Bmi-1 Regulates Extensive Erythroid Self-Renewal." Stem cell reports. 2015 Jun 9; 4(6):995-1003. Epub 2015 May 28.
Chung J, Bauer DE, Ghamari A, Nizzi CP, Deck KM, Kingsley PD, Yien YY, Huston NC, Chen C, Schultz IJ, Dalton AJ, Wittig JG, Palis J, Orkin SH, Lodish HF, Eisenstein RS, Cantor AB, Paw BH. "The mTORC1/4E-BP pathway coordinates hemoglobin production with L-leucine availability." Science signaling. 2015 8(372):ra34. Epub 2015 Apr 14.
2014 Jul 10
Niswander LM, Fegan KH, Kingsley PD, McGrath KE, Palis J. "SDF-1 dynamically mediates megakaryocyte niche occupancy and thrombopoiesis at steady state and following radiation injury." Blood. 2014 Jul 10; 124(2):277-86. Epub 2014 Apr 15.
2013 Feb 7
Kingsley PD, Greenfest-Allen E, Frame JM, Bushnell TP, Malik J, McGrath KE, Stoeckert CJ, Palis J. "Ontogeny of erythroid gene expression." Blood. 2013 Feb 7; 121(6):e5-e13. Epub 2012 Dec 12.


PhD | Developmental Biology | University of Rochester1992
MS | Developmental Biology | University of Rochester1987
BA | Biology | Reed College1982

Post-Doctoral Training & Residency

Cancer Center Fellowship in Hematopoiesis, University of Rochester School of Medicine and Dentistry, Rochester, NY 1994