Linda Marie Callahan, Ph.D.

Linda Marie Callahan, Ph.D.

Contact Information

University of Rochester Medical Center
School of Medicine and Dentistry
601 Elmwood Ave, Box 626
Rochester, NY 14642

Office: (585) 275-1317
Lab: (585) 273-2780

Lab Information

The URMC Confocal and Conventional Microscopy Core strives to provide UR researchers the ability to obtain high quality imaging data using state-of-the-art microscopy instruments.

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Research Bio

Late-onset neurodegenerative diseases, including Alzheimer's disease (AD), Amyotrophic Lateral Sclerosis (ALS), and Parkinson's Disease (PD), are the result of abnormal function of specific populations of neurons within the central nervous system. The deficits leading to clinical symptomology include a progressive synapse loss within specific neuronal networks. The synapse loss is likely mediated in part by cell loss but also by synapse dysfunction. Numerous factors have been identified as synaptic degenerators, including disruption of the cytoskeletal network, particularly of the cytoskeletal proteins mediating axonal transport.

Our primary research interests focus on axonal transport disruption mediated by cytoskeletal protein alterations. We are particularly interested in the initial response of both effected and neighboring uneffected cells to events of cytoskeletal disruption. Ongoing research focuses on the response of neurons undergoing microtubule disruption. To this end, we use primary hippocampal neurons in co-culture to examine microtubule disruption due to nocodazole (a microtubule inhibitor drug) or pathogenic tau proteins, including P301L and R406W. We are also investigating effects of overexpression of normal four-repeat tau on microtubule patterns. We are embarking on the development of late-onset models which will express tau pathogenitors at the CNS location and time of choosing of the investigator. Our analyses are multi-faceted combining histological, immunocytochemical, ultrastructural, quantitative, and molecular biological techniques to analyze alterations resulting from cytoskeletal disruption.



Long-term research goals include: 1) to determine the initial response of cell defense systems (lysosomal, ubiquitin-proteasomal-system (UPS)) to disruption of cytoskeletal proteins (microtubules and neurofilaments in particular), 2) to determine the response of cell defense systems of near neighbor neurons (apparently uneffected), 3) to determine if similarities exist between the late-onset neurodegenerative diseases of Alzheimer's Disease, Amyotrophic Lateral Sclerosis, and Parkinson's Disease, in regards to the response of neurons undergoing cytoskeletal disruption, and 4) to create models which allow control over the CNS location and age of expression of cytoskeletal disruptors. The overall goal of our research is to mimic molecular cytoskeletal disruption, and to mechanistically dissect the response of both effected and neighboring uneffected neurons with an aim towards providing therapeutic targets.

Awards & Honors (National)

Who's Who Among Students in American Universities and Colleges 1990
Invitation to Editorial Board, Journal of Neuropathology and Experimental Neurology 2004

Awards & Honors (Local)

Nominee, Student Employee Supervisor of the Year Award 1998
NIH First Award (R29) 1997
Mary Notter Award for Excellence in Research | University of Rochester Medical Center | Rochester New York 1994
Albany Medical College Alumni Association Award | Albany New York 1991
"Dean's Certificate and Prize for Excellence in Teaching", Albany Medical College Courses taught: Histology, Gross Anatomy | Albany Medical College | Albany New York 1989

Recent Journal Articles

Showing the 5 most recent journal articles. 19 available »

2013
Saul, ML; Helmreich, DL; Callahan LM.; Fudge, J. "Differences in amygdala cell proliferation between adolescent and young adult rats". Developmental Psychobiology. 2013; : DOI: 10.1002/dev.21115. Link
2012
Muyan, M; Callahan, LM; Huang Y; Lee AJ. "The ligand-mediated nuclear mobility and interaction with estrogen responsive elements of estrogen receptors are subtype-specific." J Mol Endocrinol. 2012; . Link
2010
Sharma, A*.; Callahan, L.M*.; Sul, J.Y*.; Kim, T.K.; Barrett, L.; Kim, M.; Powers, J.M.; Federoff, H.; and Eberwine, J. "A Neurotoxic Phosphoform of Elk-1 Associates with Inclusions from Multiple Neurodegenerative Diseases". PLoS ONE. 2010; 5(2): e9002. *authors contributed equally. Link
2009
Staversky, R.J.; Vitiello, P.F., Yee, M.; Callahan, L.M.; D.A. Dean, O'Reilly, M.A. "Epithelial Ablation of Bcl-XL Sensitivity to Oxygen without Disrupting Lung Development". Am J Respir Cell Mol Biol. 2009; : doi:10.1165. Link
2008
Janelsins MC, Mastrangelo MA, Park KM, Sudol KL, Narrow WC, Oddo S, LaFerla FM, Callahan LM, Federoff HJ, Bowers WJ. "Chronic neuron-specific tumor necrosis factor-alpha expression enhances the local inflammatory environment ultimately leading to neuronal death in 3xTg-AD mice." The American journal of pathology.. 2008 173(6):1768-82. Epub 2008 Oct 30.

Current Appointments

Research Assistant Professor - Department of Pathology and Laboratory Medicine (SMD) - Primary

Education

PhD | Neurobiology | Albany Medical College1991
MS | Pathology | University of Vermont1984
BS | Biology | St Lawrence University1980