Jian Fu, B.Med., Ph.D.

Jian Fu, B.Med., Ph.D.

Contact Information

Aab Cardiovascular Research Institute
211 Bailey Road
West Henrietta, NY 14586

Office: (585) 276-9855
Administrative: (585) 276-9800
Lab: (585) 276-9846
Fax: (585) 276-9830

Lab Information

Hypoxia-inducible factor a heterodimeric transcription factor composed of HIFa subunit and b/ARNT subunit. While HIF b expressed, HIF a subunit is degraded at normoxia by ubiquitin-proteasome pathway.

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Research Bio

Hypoxia-inducible factor (HIF) is a heterodimeric transcription factor composed of a HIF alpha subunit and a beta/ARNT subunit. While HIF beta subunit is constitutively expressed, HIF alpha subunit is rapidly degraded at normoxia by the ubiquitin-proteasome pathway. Importantly, it has been shown recently that the prolyl hydroxylation of HIF alpha subunit is required for its ubiquitination and subsequent destruction. The a subunit becomes hydroxylated by a family of newly identified prolyl-4-hydroxylases. Three prolyl hydroxylases namely EGLN1, 2, 3 have been identified to date.



My research focus is on elucidating of the biochemical and biological roles of SM20, the orthologue of EGLN-3, with a specific emphasis on deciphering the effect of SM20 on the differentiation and development of skeletal muscle. Towards this end, we have been using pharmalogical inhibitors to repress the activity of the prolyl hydroxylase, siRNAs to knock down SM20 expression level, and transient transfection strategy to overexpress SM20 to reveal a role for SM20 in the differentiation of cultured skeletal myoblasts. Strikingly, our preliminary results suggest that SM20 is involved in myogenesis. Our intriguing finding prompts us to dissect the underlying mechanism(s). It has been well documented that the myogenic differentiation is mainly orchestrated by a family of muscle regulatory factors (MRFs) including MyoD, myogenin, Myf-5, and MRF4. As a first step, work is in progress to explore the impact of SM20 on these master muscle-specific molecules. Further efforts will be made to identify more complicated molecular basis of SM20 on myogenesis by employing a broad variety of approaches such as (but not limited to) cDNA microarray and proteomics methodologies. In addition, we set out to create the SM20 knock-out mice to obtain more complex picture of how SM20 regulates myogenesis and development. Our study will improve the understanding of the biochemical and biological relevance of SM20 and provide novel insights into the regulation of myogenic differentiation.

Awards & Honors (Local)

Travel Award | Annual Meeting of ASBMB and Experiental Biology 2002
Paul E. Strandjord Young Investigator Award | Annual Mtg of Academy of Clinical Lab. Physicians & Scientists 2002
Guanghua Outstanding Graduate Award | Peking University 1994

Recent Journal Articles

Showing the 5 most recent journal articles. 20 available »

2010
Fu, J.; Taubman, M.B. "Prolyl hydroxylase EGLN3 regulates skeletal myoblast differentiation through an NF-kappaB-dependent pathway." J Biol Chem.. 2010; 285(12): 8927-8935.
2009
Kirby, R.J.; Jin, Y.; Fu, J.; Cubillos, J.; Swertfeger, D.; Arend, L.J. "Dynamic regulation of sphingosine-1-phosphate homeostasis during development of the mouse metanephric kidney." Am J Physiol Renal Physiol.. 2009; 296(3): F634-641.
2007 Apr 27
Fu J, Menzies K, Freeman RS, Taubman MB. "EGLN3 prolyl hydroxylase regulates skeletal muscle differentiation and myogenin protein stability." The Journal of biological chemistry.. 2007 Apr 27; 282(17):12410-8. Epub 2007 Mar 06.
2003
Fu J, Jin Y, Arend LJ. "Smac3, a novel Smac/DIABLO splicing variant, attenuates the stability and apoptosis-inhibiting activity of X-linked inhibitor of apoptosis protein." The Journal of biological chemistry.. 2003 278(52):52660-72. Epub 2003 Sep 30.
2001
Wu, X.; Zheng, J.; Zhu, JJ.; Fu, J. "Inhibitory effect of antisense VEGF121 and endostatin genes transfection on tumor growth and metastasis of human giant cell lung cancer." Chinese J Lung Cancer. 2001; 4: 83-87.

Current Appointments

Research Assistant Professor - Department of Medicine, Aab Cardiovascular Research Institute (SMD) - Primary

Education

PhD | Peking University School of Medicine1995
MD | Medicine | Tongji Medical University Yunyang Medical School1989

Post-Doctoral Training & Residency

Postdoctoral Research Associate University of Rochester School of Medicine and Dentistry (Rochester, NY) 2003
Postdoctoral Research Fellow University of Michigan Medical School (Ann Arbor, Michigan) 1999