Joseph E. Wedekind, Ph.D.

Joseph E. Wedekind, Ph.D.

Contact Information

University of Rochester Medical Center
School of Medicine and Dentistry
601 Elmwood Ave, Box 712
Rochester, NY 14642

Office: (585) 273-4516
Fax: (585) 275-6007

Lab Information

The Wedekind lab uses biophysical and biochemical techniques to investigate: (i) mechanisms of action of ncRNAs, and (ii) host protein interactions with viral factors, such as APOBEC3G and Vif.

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Research Bio

The Wedekind lab investigates the mechanisms of action of non-protein-coding (nc)RNAs. Our goal is to understand the principles by which these molecules gain biological function, which is widely applicable to human disease, as well as the identification of new antimicrobial targets. Model systems include small ribozymes derived from plant viroids, and metabolite sensing riboswitches from bacteria that regulate gene expression. This work is funded by NIH/NIGMS grant R01 GM063162.



We also study the DNA editing enzyme APOBEC3G (A3G), which is an innate anti-HIV-1 factor expressed in CD4+ T cells. Our goal is to elucidate structures of A3G in complex with HIV-1 proteins. Our long-term goal is to develop therapeutics that make A3G more potent in its ability to block viral infectivity. This work is funded by NIH/NIAID grant R33 AI076085.

Our lab primarily employs X-ray crystallography. We greatly acknowledge NIH/NCRR award S10 RR026501 for supporting this instrumentation. For access or further information see: http://dbb.urmc.rochester.edu/bcbp/facilities/bsb_facility.htm

Patents

Suppression of HIV-1 and Viral Infectivity Through Small Molecule Vif Antagonists (SMVA)

United States Serial NO.: 10/934,090
Filed Date: September 3, 2004
Title: Cytidine Deaminase Activators, Deoxycytidine Deaminase Activators, VIF Antagonists, and Methods of Screening for Molecules Thereof
Invented by: Harold Smith, Mark Sowden, Joseph Wedekind, Stephen Dewhurst, Baek Kim
RNA-Targeted Small Molecules as Potential Inhibitors of Ribosomal Frameshifting

United States Serial NO.: 12/670,772
Filed Date: July 28, 2008
Title: Nucleic Acid Binding Compounds and Methods of Use
Invented by: Benjamin Miller, Brian McNaughton, Peter Gareiss, Joseph Wedekind
Arrayed pnc-Si Membranes as Screens for Drugs That Disrupt Protein-Protein Interactions

United States Serial NO.: 12/878,700
Filed Date: September 9, 2010
Title: Drug Screening Via Nanopore Silicon Filters
Invented by: Harold Smith, James McGrath, Joseph Wedekind

Awards & Honors (National)

Editorial Board Member, The Journal of Biological Chemistry | ASBMB 2011 - Present
Executive Committee Member, CHESS Users' Group | Cornell High Energy Synchrotron Source, Ithaca NY 2010 - 2012
Elected Secretary (The Board of Directors) | The Pittsburgh Diffraction Society | Pittsburgh, PA 2007 - 2009
Life Sciences Research Foundation Fellow | Burroughs Wellcome Fund | Dept. Structural Biology, Stanford, CA 1996 - 1999
Individual NRSA Post-doctoral Fellowship (declined) | NIH | Stanford, CA 1996
Leukemia and Lymphoma Society Post-Doctoral Award (declined) | Leukemia and Lymphoma Society | Stanford, CA 1996
NIH Biophysics Training Grant Recipient | NIH/NIGMS 1992 - 1995

Awards & Honors (Local)

Faculty Teaching Award in Biophysics | University of Rochester SMD | Dept. Biochemistry & Biophysics, 2005 & 2010 2005
Dean's Fellow | Stanford University School of Medicine 1995 - 1996
Elected Member, Phi Beta Kappa | Iota Chapter of California 1990
Edmondson Summer Research Fellow | Dept. of Pathology | University of Southern California 1989

Recent Journal Articles

Showing the 5 most recent journal articles. 64 available »

2013 Sep 23
Can M, Krucinska J, Zoppellaro G, Andersen NH, Wedekind JE, Hersleth HP, Andersson KK, Bren KL. "Structural characterization of nitrosomonas europaea cytochrome c-552 variants with marked differences in electronic structure." Chembiochem : a European journal of chemical biology. 2013 Sep 23; 14(14):1828-38. Epub 2013 Jul 31.
2013 Jun
Liberman JA, Salim M, Krucinska J, Wedekind JE. "Structure of a class II preQ1 riboswitch reveals ligand recognition by a new fold." Nature chemical biology.. 2013 Jun; 9(6):353-5. Epub 2013 Apr 14.
2013 Feb 5
Wang W, Maucuer A, Gupta A, Manceau V, Thickman KR, Bauer WJ, Kennedy SD, Wedekind JE, Green MR, Kielkopf CL. "Structure of phosphorylated SF1 bound to U2AF?? in an essential splicing factor complex." Structure (London, England : 1993). 2013 Feb 5; 21(2):197-208. Epub 2012 Dec 27.
2013
Suddala KC, Rinaldi AJ, Feng J, Mustoe AM, Eichhorn CD, Liberman JA, Wedekind JE, Al-Hashimi HM, Brooks CL, Walter NG. "Single transcriptional and translational preQ1 riboswitches adopt similar pre-folded ensembles that follow distinct folding pathways into the same ligand-bound structure." Nucleic acids research.. 2013 41(22):10462-75. Epub 2013 Sep 03.
2012 Nov 6
Salter JD, Lippa GM, Belashov IA, Wedekind JE. "Core-binding factor ? increases the affinity between human Cullin 5 and HIV-1 Vif within an E3 ligase complex." Biochemistry.. 2012 Nov 6; 51(44):8702-4. Epub 2012 Oct 25.

Current Appointments

Professor - Department of Biochemistry and Biophysics (SMD) - Primary
Associate Professor - Cancer Center

Education

Ph.D. | Biochemistry (Structural Enzymology) | University of Wisconsin at Madison1995
B.Sc. | Biochemistry (Magna Cum Laude) | University of California at Riverside1990

Post-Doctoral Training & Residency

RNA Biophysics, Dept. of Structural Biology, Stanford University School of Medicine. 1995-1999 1999